A reliable phenotypic assay for detection of ESBLs and AmpCs in MBL-producing gram-negative bacteria with the use of aminophenylboronic acid, dipicolinic acid and cloxacillin

Autor:	Shravani Mitra, Somdatta Chatterjee, Sulagna Basu, Saswati Datta
Rok vydání:	2015
Předmět:	Microbiology (medical) Gram-negative bacteria Cefotaxime Microbiology beta-Lactamases chemistry.chemical_compound Cloxacillin Bacterial Proteins Clavulanic acid Gram-Negative Bacteria polycyclic compounds medicine Humans Cefoxitin Phenylboronic acid Picolinic Acids Molecular Biology Chromatography biology biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Dipicolinic acid Boronic Acids Anti-Bacterial Agents Bacterial Typing Techniques Mueller-Hinton agar Phenotype chemistry bacteria Gram-Negative Bacterial Infections geographic locations medicine.drug
Zdroj:	Journal of Microbiological Methods. 115:100-103
ISSN:	0167-7012
Popis:	ESBLs and AmpCs may escape detection when they coexist with metallo-β-lactamases such as New Delhi Metallo-β-lactamases-1. In this study a combination disk assay was established using cefotaxime, cefotaxime/clavulanic acid, cefotaxime/clavulanic acid/cloxacillin, cefoxitin and cefoxitin/phenylboronic acid/cloxacillin on Mueller Hinton agar supplemented with dipicolinic acid for determination of β-lactamases in the presence of NDM-1.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fba5676af0da5e017b5704153ca2da9b https://doi.org/10.1016/j.mimet.2015.05.028 Zobrazit plný text záznamu Full Text from ScienceDirect