Differentiating brown and white adipose tissues by high-resolution diffusion NMR spectroscopy
| Autor: | Swee Shean Lee, Navin Michael, Kaz Nagashima, Rengaraj Anantharaj, S. Sendhil Velan, Suresh Anand Sadananthan, Sanjay Kumar Verma, Venkatesh Gopalan, Tian Xianfeng, Jadegoud Yaligar |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Magnetic Resonance Spectroscopy diffusion spectroscopy Diffusion Adipose Tissue White lipid droplet Adipose tissue QD415-436 White adipose tissue Diet High-Fat Biochemistry 030218 nuclear medicine & medical imaging magnetic resonance 03 medical and health sciences 0302 clinical medicine Endocrinology white adipose tissue Adipose Tissue Brown In vivo Internal medicine Lipid droplet Brown adipose tissue medicine Methods Effective diffusion coefficient Animals Humans Obesity chemistry.chemical_classification Chemistry Fatty acid food and beverages nutritional and metabolic diseases brown adipose tissue Cell Biology adipose tissue Rats 030104 developmental biology medicine.anatomical_structure lipids (amino acids peptides and proteins) Energy Metabolism |
| Zdroj: | Journal of Lipid Research, Vol 58, Iss 1, Pp 289-298 (2017) |
| ISSN: | 1539-7262 |
| Popis: | There are two types of fat tissues, white adipose tissue (WAT) and brown adipose tissue (BAT), which essentially perform opposite functions in whole body energy metabolism. There is a large interest in identifying novel biophysical properties of WAT and BAT by a quantitative and easy-to-run technique. In this work, we used high-resolution pulsed field gradient diffusion NMR spectroscopy to study the apparent diffusion coefficient (ADC) of fat molecules in rat BAT and WAT samples. The ADC of fat in BAT and WAT from rats fed with a chow diet was compared with that of rats fed with a high-fat diet to monitor how the diffusion properties change due to obesity-associated parameters such as lipid droplet size, fatty acid chain length, and saturation. Feeding a high-fat diet resulted in increased saturation, increased chain lengths, and reduced ADC of fat in WAT. The ADC of fat was lower in BAT relative to WAT in rats fed both chow and high-fat diets. Diffusion of fat was restricted in BAT due to the presence of small multilocular lipid droplets. Our findings indicate that in vivo diffusion might be a potential way for better delineation of BAT and WAT in both lean and obese states. |
| Databáze: | OpenAIRE |
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