MicroRNA-136 functions as a tumor suppressor in osteosarcoma via regulating metadherin

Autor:	Tao Guo, Guobiao Pan
Rok vydání:	2018
Předmět:	0301 basic medicine Adult Male Cancer Research Bone Neoplasms Biology medicine.disease_cause law.invention 03 medical and health sciences Young Adult 0302 clinical medicine law Cell Movement microRNA Genetics medicine Humans Genes Tumor Suppressor Neoplasm Invasiveness Cell Proliferation Osteosarcoma Cell growth Cancer Membrane Proteins RNA-Binding Proteins MTDH General Medicine medicine.disease MicroRNAs 030104 developmental biology Oncology Cell culture 030220 oncology & carcinogenesis Cancer research Suppressor Female Carcinogenesis Cell Adhesion Molecules
Zdroj:	Cancer biomarkers : section A of Disease markers. 22(1)
ISSN:	1875-8592
Popis:	BACKGROUND Accumulating studies have reported the abnormal expression of microRNA-136 (miR-136) in numerous types of human cancer, and its involvement in cancer initiation and progression. However, there are no investigations of miR-136 in osteosarcoma (OS). OBJECTIVE To explore the expression pattern, clinical significance and potential roles of miR-136 in OS. METHODS miR-136 expression in clinical OS tissues and human OS cell lines were detected by qPCR, and its associations with clinicopathological characteristics of OS patients were statistically analyzed. Then, the effects of miR-136 on OS cell proliferation, migration and invasion were assessed in vitro. Its underling mechanisms were also investigated. RESULTS miR-136 expression in OS tissues and cells were dramatically decreased compared with corresponding non-cancerous tissues and cells, respectively. Low miR-136 expression was significantly associated with aggressive clinical features, including the advanced clinical stage, the presence of lung and distant metastasis (all P< 0.05). Additionally, enforced expression of miR-136 obviously inhibited the proliferation, migration and invasion of OS cells in vitro. Mechanistically, metadherin (MTDH) was predicted and verified as a target gene of miR-136. Further functional experiments indicated that the loss of MTDH abrogated the tumor suppressive roles of miR-136 in OS cells. CONCLUSION Our findings provide the first evidence that the aberrant expression of miR-136 may be implicated into carcinogenesis and cancer progression of OS. Functionally, miR-136 may inhibit the proliferation, migration and invasion of OS cells via negatively regulating its target gene MTDH. Thus, miR-136-MTDH axis may be a potential therapeutic targets for the treatment of OS.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fba3fce9fdfe41202a942232d3fc02f3 https://pubmed.ncbi.nlm.nih.gov/29562498 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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