Application of the Gyrolab microfluidic platform to measure picomolar affinity of a PD-L1-binding Adnectin™ radioligand for positron emission tomography
| Autor: | Juhi Juneja, Zhou Dai, Daniel Cohen, Lumelle A. Schneeweis, Ruchira Dasgupta, Frank Marsilio, Paul E. Morin |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
medicine.diagnostic_test Chemistry Microfluidics Protein engineering Ligands General Biochemistry Genetics and Molecular Biology B7-H1 Antigen 03 medical and health sciences 0302 clinical medicine Positron emission tomography 030220 oncology & carcinogenesis Positron-Emission Tomography medicine Radioligand Biophysics Humans 030304 developmental biology Biotechnology Protein Binding |
| Zdroj: | BioTechniques. 69(3) |
| ISSN: | 1940-9818 |
| Popis: | Advances in in vitro display and protein engineering yield therapeutics with affinities in the picomolar range. The Gyrolab® microfluidics platform uses the kinetic exclusion assay principle to measure subnanomolar solution affinities. This work describes application of the Gyrolab solution affinity module and the new multi-curve analysis feature to determine affinity of the PD-L1 Adnectin™ positron emission tomography radioligand, which was measured as 20 pM for human PD-L1. We also report key parameters that affect assay signal-to-background ratio and data quality, such as detection reagent concentration. Gyrolab offers the necessary throughput for rapid assay development with low sample consumption, as demonstrated in this study, which also provides helpful tips for assay optimization for solution affinity measurement. |
| Databáze: | OpenAIRE |
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