Phenotype markers and function of neutrophils in children with hemolytic uremic syndrome
| Autor: | Paula Barrionuevo, Carolina Rubel, Martín A. Isturiz, Marina S. Palermo, Flavia B Ramírez, Mario Diaz, Gabriela C. Fernández, Laura Cecilia López |
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| Rok vydání: | 2002 |
| Předmět: |
Hemolytic anemia
Nephrology Adult Male medicine.medical_specialty Adolescent Renal glomerulus Neutrophils medicine.medical_treatment Macrophage-1 Antigen urologic and male genital diseases Cell Degranulation Reference Values Internal medicine medicine Humans Child Dialysis Uremia business.industry Elastase Cell Membrane Receptors IgG Antibody-Dependent Cell Cytotoxicity medicine.disease Pathophysiology Phenotype Pediatrics Perinatology and Child Health Immunology Chronic Disease Hemolytic-Uremic Syndrome Tumor necrosis factor alpha Female business Biomarkers Kidney disease |
| Zdroj: | Pediatric nephrology (Berlin, Germany). 17(5) |
| ISSN: | 0931-041X |
| Popis: | The hemolytic uremic syndrome (HUS) is the most-common cause of acute renal failure in children. Several researchers have reported the presence of neutrophil (PMN) activating cytokines, such as interleukin-8 and tumor necrosis factor-alpha, in the sera of HUS patients. Moreover, PMN-derived products, such as elastase, were increased. These observations have lead to the hypothesis that activated PMN could act as mediators of endothelial damage. The objective of this investigation was to directly evaluate the activation status of peripheral PMN from children with HUS. For this purpose, 12 children with typical HUS were bled during the acute period, before dialysis and/or transfusion, and 8 of them were also bled after 1 month follow-up. Additionally, blood samples from healthy control children admitted for routine surgical procedures, chronic uremic children, and neutrophilic children with acute infections not related to HUS were collected and processed in an identical manner. The function and membrane activation markers of PMN from these groups were evaluated. We found that during the acute period of HUS, PMN had reduced expression of FcgammaRIII (CD16) and CD11b, were degranulated, and exhibited an impaired antibody-dependent cellular cytotoxicity. These parameters returned to normal after clinical recuperation. We conclude that PMN activation in HUS patients is a very early and transient event, and upon hospitalization before dialysis PMN show a phenotype and functional pattern of partial deactivation. |
| Databáze: | OpenAIRE |
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