K+ Channels and Guinea-pig Trachea: a Possible Functional Modulation by GABAB Receptors
| Autor: | Bruno D'Agostino, M. Costantino, Francesca Rossi, M. Mangrella, Maria Gabriella Matera, V. Calderaro |
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| Přispěvatelé: | Matera, Maria Gabriella, D'Agostino, Bruno, Costantino, M, Mangrella, M, Calderaro, V, Rossi, F. |
| Rok vydání: | 1994 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Cromakalim medicine.medical_specialty Potassium Channels Muscle Relaxation Guinea Pigs Neuromuscular Junction Stimulation GABAB receptor chemistry.chemical_compound Phaclofen Internal medicine medicine Animals Benzopyrans Pyrroles Pharmacology (medical) General Pharmacology Toxicology and Pharmaceutics Neurotransmitter Receptor Tetraethylammonium Hyperpolarization (biology) Electric Stimulation Bronchodilator Agents Trachea Endocrinology Receptors GABA-B chemistry Biophysics Acetylcholine medicine.drug |
| Zdroj: | Pulmonary Pharmacology. 7:259-263 |
| ISSN: | 0952-0600 |
| DOI: | 10.1006/pulp.1994.1029 |
| Popis: | Summary: K + channel activators represent a novel class of smooth muscle relaxant agents. There is now much evidence demonstrating that K + channels, localized to prejunctional neurones and post-junctional smooth muscle membranes, can regulate airway smooth muscle activity, inducing smooth muscle cell membrane hyperpolarization. K + channel activity may be influenced by some neurotransmitters, such as adenosine, serotonin and noradrenaline. More recently, it has been observed that the stimulation of GABA B receptors influences K + channels in the hippocampus, dorsal rafe and spinal cord neurons. The aim of this study was to investigate the effects of levcromakalim in guinea-pig trachea at pre- and post-junctional sites and to evaluate whether GABA B receptors may modulate K + channel activation. Levcromakalim (from 1 nM to 1 μM) relaxed guinea-pig trachea (IC 50 10±0.9 nM) previously contracted by KCl (30 mM). This effect was reversed by a pretreatment with tetraethylammonium (10 mM) (IC 50 120±0.7 nM). A 30-min pretreatment with baclofen (1 μM) or phaclofen (1 μM) failed to modify the effects of levcromakalim (IC 50 18±1.0 nM and 14±0.6 nM, respectively). The contractile responses to electrical field stimulation (71.20±5.12% of acetylcholine -100 μM-contraction) was significantly ( P P P + channels may be localized both to prejunctional and post-junctional post-ganglionic sites, and may play an important role in modulating airway responsiveness. In fact, they may act at the prejunctional neurones, inhibiting the release of contractile neurotransmitter, or at the post-junctional sites, hyperpolarizing smooth muscle airway membranes. Moreover, the present study seems to demonstrate that K + channels localized on the prejunctional post-ganglionic nerves may be influenced by GABA B receptors. |
| Databáze: | OpenAIRE |
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