Comparison of inherently essential genes ofPorphyromonas gingivalisidentified in two transposon-sequencing libraries
| Autor: | Himabindu Gogeneni, Richard J. Lamont, Justin A Hutcherson, David A. Scott, Erik L. Hendrickson, Marvin Whiteley, Deborah R. Yoder-Himes, Murray Hackett |
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| Rok vydání: | 2015 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine Microbiology (medical) Transposable element Immunology Mutant Mutagenesis (molecular biology technique) Sigma Factor Microbiology Article 03 medical and health sciences Bacterial Proteins Sigma factor Genomic library KEGG General Dentistry Gene Porphyromonas gingivalis Heat-Shock Proteins Periodontal Diseases Gene Library Genetics Genes Essential biology Chromosome Mapping High-Throughput Nucleotide Sequencing biology.organism_classification Mutagenesis Insertional Pyrimidines 030104 developmental biology Genes Bacterial Mutation DNA Transposable Elements |
| Zdroj: | Molecular Oral Microbiology. 31:354-364 |
| ISSN: | 2041-1006 |
| DOI: | 10.1111/omi.12135 |
| Popis: | Porphyromonas gingivalis is a Gram-negative anaerobe and keystone periodontal pathogen. A mariner transposon insertion mutant library has recently been used to define 463 genes as putatively essential for the in vitro growth of P. gingivalis ATCC 33277 in planktonic culture [Library 1, (Klein et al 2012)]. We have independently generated a transposon insertion mutant library [Library 2] for the same P. gingivalis strain and herein compare genes that are putatively essential for in vitro growth in complex media, as defined by both libraries. 281 genes (61%) identified by Library 1 were common to Library 2. Many of these common genes are involved in fundamentally important metabolic pathways, notably pyrimidine cycling as well as lipopolysaccharide, peptidoglycan, pantothenate and coenzyme A biosynthesis and nicotinate and nicotinamide metabolism. Also in common are genes encoding heat shock protein homologues, sigma factors, enzymes with proteolytic activity and the majority of sec-related protein export genes. In addition to facilitating a better understanding of critical physiological processes, transposon sequencing technology has the potential to identify novel strategies for the control of P. gingivalis infections. Those genes defined as essential by two independently generated TnSeq mutant libraries are likely to represent particularly attractive therapeutic targets. |
| Databáze: | OpenAIRE |
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