IL-2-induced IL-9 production by allergen-specific human helper T-cell clones
| Autor: | Yuichiro Kajiyama, Makiko Umezu-Goto, Noriaki Kobayashi, Kazuhisa Takahashi, Yoshinosuke Fukuchi, Akio Mori |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
CD3 Complex
Immunology Lymphocyte Activation CD28 Antigens Immunology and Allergy Animals Humans Antigens Dermatophagoides Calcium Signaling Cells Cultured Interleukin-13 Dermatophagoides farinae Dose-Response Relationship Drug Tissue Extracts Ionomycin Interleukin-9 Antibodies Monoclonal Receptors Interleukin-2 General Medicine T-Lymphocytes Helper-Inducer Allergens Asthma Clone Cells Up-Regulation Receptor-CD3 Complex Antigen T-Cell Interleukin-2 Tetradecanoylphorbol Acetate Interleukin-5 Muromonab-CD3 |
| Zdroj: | International archives of allergy and immunology. 143 |
| ISSN: | 1423-0097 |
| Popis: | Background: IL-9 is an important cytokine in allergic diseases such as asthma, atopic dermatitis, etc. T helper (Th) cells seem to be the main source of IL-9. Cellular and molecular mechanisms of IL-9 production by human Th cells have been poorly understood. Methods:Dermatophagoides farinae(Der f)-specific Th clones were established from peripheral blood lymphocytes of atopic asthmatics, and cytokine synthesis in response to various stimuli was determined by specific ELISAs. Results: IL-9 was produced by 14 of 27 human Th clones upon T cell receptor (TCR) stimulation, immobilized anti-CD3 antibody (Ab). IL-9 production was significantly enhanced by the addition of anti-CD28 Ab into the culture, indicating the role of costimulatory signal on IL-9 synthesis. Pharmacologically, IL-9 production was induced by ionomycin (IOM) alone, and enhanced by phorbol 12-myristate 13-acetate (PMA). rIL-2 induced IL-9 production by 8 out of 19 Th clones. IL-9 production by Th clones stimulated with immobilized anti-CD3 Ab was significantly suppressed by the addition of anti-IL-2 neutralizing Ab into the culture. Conclusion: Approximately half of the Der f-specific Th clones derived from atopic asthmatics produced IL-9 upon TCR stimulation. Ca2+ signal, CD28 signal, and IL-2 receptor signal seem to play important roles on IL-9 production by human Th cells. Moreover, synthesis of IL-9, a Th2 cytokine, is dependent on IL-2, a Th1 cytokine, which is produced by Th cells themselves. |
| Databáze: | OpenAIRE |
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