Two-component mediated peroxide sensing and signal transduction in fission yeast
| Autor: | Jill Cheetham, Vicky Buck, Brian A. Morgan, Panagiota Malakasi, Janet Quinn, Jonathan B. A. Millar, Deborah A. Smith |
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| Rok vydání: | 2010 |
| Předmět: |
Histidine Kinase
Physiology Clinical Biochemistry Biochemistry Schizosaccharomyces Phosphorylation Protein kinase A Molecular Biology Transcription factor Histidine General Environmental Science biology Kinase Histidine kinase Cell Biology Hydrogen Peroxide biology.organism_classification Cell biology DNA-Binding Proteins Response regulator Microscopy Fluorescence Schizosaccharomyces pombe General Earth and Planetary Sciences Schizosaccharomyces pombe Proteins Signal transduction Mitogen-Activated Protein Kinases Protein Kinases Signal Transduction Transcription Factors |
| Zdroj: | Antioxidantsredox signaling. 15(1) |
| ISSN: | 1557-7716 |
| Popis: | Two-component related proteins play a major role in regulating the oxidative stress response in the fission yeast, Schizosaccharomyces pombe. For example, the peroxide-sensing Mak2 and Mak3 histidine kinases regulate H(2)O(2)-induced activation of the Sty1 stress-activated protein kinase pathway, and the Skn7-related response regulator transcription factor, Prr1, is essential for activation of the core oxidative stress response genes. Here, we investigate the mechanism by which the S. pombe two-component system senses H(2)O(2), and the potential role of two-component signaling in the regulation of Prr1. Significantly, we demonstrate that PAS and GAF domains present in the Mak2 histidine kinase are essential for redox-sensing and activation of Sty1. In addition, we find that Prr1 is required for the transcriptional response to a wide range of H(2)O(2) concentrations and, furthermore, that two-component regulation of Prr1 is specifically required for the response of cells to high levels of H(2)O(2). Significantly, this provides the first demonstration that the conserved two-component phosphorylation site on Skn7-related proteins influences resistance to oxidative stress and oxidative stress-induced gene expression. Collectively, these data provide new insights into the two-component mediated sensing and signaling mechanisms underlying the response of S. pombe to oxidative stress. |
| Databáze: | OpenAIRE |
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