Use of Flow Cytometry for Producing Tetraploids in Red Clover

Autor: Dace Grauda, Aija Rebāne, Aldis Jansons, Isaak Rashal, Sarmīte Rancāne
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Proceedings of the Latvian Academy of Sciences. Section B, Natural Sciences, Vol 74, Iss 5, Pp 344-346 (2020)
Popis: Sprouts of diploid red clover ‘Stendes Agrais’ were treated with 0.2% colchicine solution. The ploidy level of plants was detected by flow cytometry. Most of the treated plants were mixoploids and sterile. From progenies of fertile plants, mixoploids were selected that had a proportion of tetraploid cells higher than 60%. In the next generation, stable tetraploid plants were selected, and 49 lines were included in the breeding process.
Databáze: OpenAIRE