Epidermal growth factor receptor and proliferating cell nuclear antigen expression in urine ThinPrep specimens
| Autor: | D. Alexopoulou, Helen Koutselini, V. Kyriakidou, Ekaterini Politi, S. Lambropoulou, Andreas C. Lazaris |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Histology Cytodiagnosis Urine Malignancy Pathology and Forensic Medicine Predictive Value of Tests Proliferating Cell Nuclear Antigen Humans Medicine Epidermal growth factor receptor Stage (cooking) Aged Neoplasm Staging Urine cytology Aged 80 and over Bladder cancer biology medicine.diagnostic_test business.industry Reproducibility of Results General Medicine Middle Aged medicine.disease Immunohistochemistry Proliferating cell nuclear antigen ErbB Receptors Urinary Bladder Neoplasms biology.protein Female business |
| Zdroj: | Cytopathology. 16:303-308 |
| ISSN: | 1365-2303 0956-5507 |
| DOI: | 10.1111/j.1365-2303.2005.00270.x |
| Popis: | Objective: To evaluate proliferating cell nuclear antigen (PCNA) and epidermal growth factor receptor (EGFR) expression in urine ThinPrep (TP) specimens, to compare these findings with clinical and histological features and to determine whether these immunomarkers are predictive of clinical stage. Patients and Methods: The TP processed urine samples and the corresponding tissue sections from 42 patients with newly diagnosed bladder cancer (18 non-muscle invasive and 24 muscle invasive) were included in our study. Urine was collected for cytological evaluation before transurethral resection. Tumour grade and clinical stage were assessed from the transurethral resection specimens. The EGFR and PCNA expression was obtained by an automated immunostainer. Results: There was a remarkable concordance in the expression of both antibodies in TP smears and tissue sections. No significant association was detected for any of the immunomarkers examined with regard to tumour grade. The EGFR expression as well as grade of malignancy were significantly associated with stage of disease (P = 0.0001). PCNA was not found to be a significant predictor of stage (P = 0.210). Conclusion: Our data suggest that the evaluation of grade of malignancy and EGFR immunopositivity can be considered as reliable predictors of disease stage in urine TP specimens. |
| Databáze: | OpenAIRE |
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