Induction of Apoptosis by a Combination of Paclitaxel and Carboplatin in the Presence of Hyperthermia
| Autor: | Chun-Ping Zou, Xu-Hui Li, Xiucheng Li, Guangjian Jiang, Weihua Gong, Tao Huang, Dian-Peng Feng |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Hyperthermia
Cancer Research Fever Paclitaxel Epidemiology Apoptosis Bone Neoplasms Pharmacology Carboplatin Flow cytometry Immunoenzyme Techniques chemistry.chemical_compound Antineoplastic Combined Chemotherapy Protocols Tumor Cells Cultured medicine Humans MTT assay fas Receptor Osteosarcoma medicine.diagnostic_test Chemistry Public Health Environmental and Occupational Health medicine.disease Combined Modality Therapy Oncology Cell culture |
| Zdroj: | Asian Pacific Journal of Cancer Prevention. 13:81-85 |
| ISSN: | 1513-7368 |
| Popis: | Purpose: To study enhancing effects of paclitaxel in the thermochemotherapy of osteosarcoma cell lines and related mechanisms. Materials and Methods: Paclitaxel and carboplatin were used alone or jointly on OS732 cell lines in the presence of hyperthermia. Inhibition of proliferation was measured by MTT assay and cellular changes were assessed with inverted phase contrast and fluorescence microscopy. Apoptosis was analyzed with flow cytometry (FCM) and Fas expression by immunocytochemistry. Results: At 43℃, one hour after the application of 10ug/ml paclitaxel and 5mg/ml carboplatin on OS732 cells jointly, the survival rate was 15.8% which was significantly lower than with 10 mg/ml paclitaxel (45.8%) and 5mg/ml carboplatin (47.7%) respectively (P |
| Databáze: | OpenAIRE |
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