Human Adipose-Derived and Amniotic Fluid-Derived Stem Cells: A Preliminary In Vitro Study Comparing Myogenic Differentiation Capability
| Autor: | Pawel Sadlecki, Marek Grabiec, Krzysztof Roszkowski, Joanna Olkowska, Dorota Porowińska, Tomasz Drewa, Anna Bajek, Malgorzata Walentowicz-Sadlecka |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult Cell type Adolescent CD34 Adipose tissue Pilot Projects Biology Muscle Development 03 medical and health sciences SOX2 Lab/In Vitro Research Myocyte Humans Regeneration CD90 Cell Lineage Muscle Skeletal Cells Cultured Adiposity Cell Proliferation Muscle Cells Regeneration (biology) Stem Cells Cell Differentiation General Medicine Middle Aged Cell Dedifferentiation Amniotic Fluid Cell biology 030104 developmental biology Adipose Tissue Female Stem cell |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| Popis: | BACKGROUND Around the world, disabilities due to musculoskeletal disorders have increased and are a major health problem worldwide. In recent years, stem cells have been considered to be powerful tools for musculoskeletal tissue engineering. Human adipose-derived stem cells (hADSCs) and amniotic fluid-derived stem cells (hAFSCs) undergo typical differentiation process into cells of mesodermal origin and can be used to treat muscular system diseases. The aim of the present study was to compare the biological characteristic of stem cells isolated from different human tissues (adipose tissue and amniotic fluid) with respect to myogenic capacity and skeletal and smooth muscle differentiation under the same conditions. MATERIAL AND METHODS hAFSCs and hADSCs were isolated during standard medical procedures and widely characterized by specific markers expression and differentiation potential. Both cell types were induced toward smooth and striated muscles differentiation, which was assessed with the use of molecular techniques. RESULTS For phenotypic characterization, both stem cell types were assessed for the expression of OCT-4, SOX2, CD34, CD44, CD45, and CD90. Muscle-specific markers appeared in both stem cell types, but the proportion of positive cells showed differences depending on the experimental conditions used and the source from which the stem cells were isolated. CONCLUSIONS In this study, we demonstrated that hADSCs and hAFSCs have different capability of differentiation toward both muscle types. However, hADSCs seem to be a better source for myogenic protocols and can promote skeletal and smooth muscle regeneration through either direct muscle differentiation or by paracrine mechanism. |
| Databáze: | OpenAIRE |
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