Autophagosome activity in macrophage for atherosclerotic plaques in ApoE−/− mice enhanced by Tiaozhi Tongmai Granules
| Autor: | Shuwen Guo, Zhaoduo Su, Xi Chen, Min Zheng, Jiani Wu, Yan Tang, Lu Zhang, Qing Sun, Wenchen Liu, Chenglong Zheng, Jiangong Wu |
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| Rok vydání: | 2015 |
| Předmět: |
Autophagosome
Pathology medicine.medical_specialty Chinese medicine LAMP1 medicine.diagnostic_test System pharmacology Atorvastatin Immunofluorescence Autophagy Colocalization Biology Pharmacology lcsh:RZ409.7-999 Atherosclerosis Pathophysiology Staining Complementary and alternative medicine medicine lcsh:Miscellaneous systems and treatments medicine.drug |
| Zdroj: | Journal of Traditional Chinese Medical Sciences, Vol 2, Iss 3, Pp 194-204 (2015) |
| ISSN: | 2095-7548 |
| DOI: | 10.1016/j.jtcms.2016.03.002 |
| Popis: | Objective: Atherosclerosis is the fundamental pathophysiologic component of cardiovascular disease, and Tiaozhi Tongmai Granules show great efficiency in the treatment of the disease. However, the mechanism of Tiaozhi Tongmai Granules is still unclear. In this study, we have combined experiments with network pharmacology to explore the anti-atherosclerosis mechanism of Tiaozhi Tongmai Granules. Method: 120 male ApoE−/− mice were randomly divided into three groups: the model group, Chinese herb group and Atorvastatin group. The model group, Atorvastatin group and Chinese herb group were fed with a high-fat diet, a high-fat diet plus atorvastatin (5.1 mg/kg/d) and a high-fat diet plus Tiaozhi Tongmai Granules (16.5 g/kg/d) for 16 weeks, respectively. Atherogenesis was identified by H&E staining. The colocalization of neutral lipid stain BODIPY and microtubule-associated protein 1 light chain 3 (LC3) and the colocalization of BODIPY and lysosomal-associated membrane protein 1 (LAMP1) within ApoE−/− mice aortic plaques were tested using fluorescence confocal microscopy and the Pearson's coefficients were calculated. To further explore the anti-atherosclerosis mechanism of Tiaozhi Tongmai Granules, the network pharmacology was used to construct the herb-compound-target network. Results: The size of the aortic lipid plaque in the Chinese herb group and Atorvastatin group were smaller compared with the model group on the 16th week. Compared with the model group on the 16th week, the BODIPY and LC3 colocalization rate, the BODIPY and LAMP1 colocalization rate of the Chinese herb group and Atorvastatin group all presented significant increase in the aortic plaque (P |
| Databáze: | OpenAIRE |
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