E2F3 transcription factor: A promising biomarker in lung cancer
| Autor: | Hala Abdel Al Ahmed, Ola H. Nada |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Adult
Male 0301 basic medicine Cancer Research Pathology medicine.medical_specialty Lung Neoplasms Disease Pathogenesis 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Biomarkers Tumor Genetics Humans Medicine Clinical significance RNA Messenger Lung cancer Messenger RNA Lung business.industry General Medicine Middle Aged respiratory system Prognosis medicine.disease respiratory tract diseases Gene Expression Regulation Neoplastic 030104 developmental biology Real-time polymerase chain reaction medicine.anatomical_structure Oncology E2F3 Transcription Factor 030220 oncology & carcinogenesis Cancer research Biomarker (medicine) Female business |
| Zdroj: | Cancer Biomarkers. 19:21-26 |
| ISSN: | 1875-8592 1574-0153 |
| DOI: | 10.3233/cbm-160196 |
| Popis: | Background Many researches aiming to explore the pathogenesis of lung cancer have extensively studied the molecular alteration in such disease. Objective In the present study we measured the blood E2F3 mRNA using real-time RT-PCR technique in order to evaluate its clinical significance in early diagnosis and monitoring of lung cancer. Methods This case-control study included 50 lung cancer patients, 20 patients with benign lung diseases and 20 healthy controls. Relative quantification of blood E2F3 mRNA was done by real-time RT-PCR. Results Blood E2F3 mRNA levels were significantly higher in lung cancer patients when compared to either patients with benign lung diseases or healthy subjects. This elevation was significant in those with metastatic lung cancer as compared to those with localized lung cancer. At a cutoff^{(2-Δ Δ CT)} 1.5, blood E2F3 mRNA was able to distinguish malignant from benign lung conditions with a diagnostic sensitivity of 100%; while at a cutoff^{(2-Δ Δ CT)} 5.3, blood E2F3 mRNA discriminated localized from metastatic lung cancer with a sensitivity of 93.6%. Conclusions Blood E2F3 mRNA is a sensitive diagnostic marker in lung cancer; moreover, it is a promising prognostic marker capable of efficiently discriminating early from late stages of the disease. |
| Databáze: | OpenAIRE |
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