Characterisation of porcine enteropathogenic Escherichia coli isolated in northeastern India
| Autor: | Rajkumari Mandakini, P. K. Subudhi, Parimal Roychoudhury, Tapan Kumar Dutta, Jonathan Lalsiamthara, Lalhruaipuii, Hosterson Kylla |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Serotype Veterinary medicine 040301 veterinary sciences Sequence analysis 030106 microbiology Virulence Drug resistance Biology piglets digestive system 0403 veterinary science 03 medical and health sciences epec parasitic diseases SF600-1100 medicine Enteropathogenic Escherichia coli drug resistance General Veterinary Molecular epidemiology 04 agricultural and veterinary sciences Amoxicillin biochemical phenomena metabolism and nutrition bacterial infections and mycoses Breed virulence serotypes bacteria medicine.drug Research Article |
| Zdroj: | Journal of Veterinary Research, Vol 64, Iss 3, Pp 391-397 (2020) Journal of Veterinary Research |
| ISSN: | 2450-8608 |
| Popis: | Introduction Enteropathogenic Escherichia coli (EPEC) is one of the main pathotypes causing gastroenteritis, particularly in young immunocompromised hosts. The study reports the prevalence, characterisation, and molecular epidemiology of EPEC from piglets in northeastern India. Material and Methods A total of 457 faecal samples were collected, from which 1,286 E. coli strains were isolated and screened by PCR. The resultant EPEC strains were serotyped and phenotypically characterised for resistance against 15 antimicrobials. Also, the phylogenetic sequence was analysed for 11 selected strains. Results A total of 42 strains (3.26%) belonged to atypical EPEC, of which, 15 (35.71%, and 2.29% of the 654 strains from this farm type) were isolated from organised and 27 (64.29%, and 4.27% of the 632 strains from this farm type) from unorganised farms; further, 5 (11.90% of the EPEC strains and 1.51% of the 330 strains from this breed) were isolated from the indigenous breeds and 37 (88.10%, and 3.87% of the 956 strains from this breed) from crossbred piglets. Serogroups O111 (11.9%) and O118 (7.14%) were the most prevalent of the 10 present. Sequence analysis of a length of the eaeA gene of 11 isolates of the region showed them to have 100% homology with each other and their identity ranged from 99.4% to 99.7% with GenBank reference sequences. All the EPEC isolates were multi-drug resistant, showing the highest resistance to amoxicillin (80.9%) and cephalexin (76.19%). Conclusion The study highlighted the association of EPEC with piglet’s diarrhoea in northeastern India. EPEC isolates belonged to many serotypes and phenotypically all were multi-drug resistant with close genetic homology. |
| Databáze: | OpenAIRE |
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