LHP1 Interacts with ATRX through Plant-Specific Domains at Specific Loci Targeted by PRC2
| Autor: | Elin Axelsson, Sean A. Montgomery, Bas J. G. E. Pieters, Abbas H. K. Al Temimi, Jasmin Mecinović, Sarah Holec, Danhua Jiang, Zdravko J. Lorković, Frédéric Berger, Haifeng Wang |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine animal structures Chromosomal Proteins Non-Histone Arabidopsis Synthetic Organic Chemistry Plant Science 01 natural sciences Histones 03 medical and health sciences Constitutive heterochromatin Arabidopsis thaliana Molecular Biology ATRX biology Arabidopsis Proteins fungi Polycomb Repressive Complex 2 biology.organism_classification Chromatin Cell biology Repressor Proteins 030104 developmental biology Histone Chromobox Protein Homolog 5 biology.protein Heterochromatin protein 1 PRC2 010606 plant biology & botany |
| Zdroj: | Molecular Plant, 11, 1038-1052 Molecular Plant, 11, 8, pp. 1038-1052 |
| ISSN: | 1674-2052 |
| Popis: | Heterochromatin Protein 1 (HP1) is a major regulator of chromatin structure and function. In animals, the network of proteins interacting with HP1 is mainly associated with constitutive heterochromatin marked by H3K9me3. HP1 physically interacts with the putative ortholog of the SNF2 chromatin remodeler ATRX, which controls deposition of histone variant H3.3 in mammals. In this study, we show that the Arabidopsis thaliana ortholog of ATRX participates in H3.3 deposition and possesses specific conserved domains in plants. We found that plant Like HP1 (LHP1) protein interacts with ATRX through domains that evolved specifically in land plant ancestors. Loss of ATRX function in Arabidopsis affects the expression of a limited subset of genes controlled by PRC2 (POLYCOMB REPRESSIVE COMPLEX 2), including the flowering time regulator FLC. The function of ATRX in regulation of flowering time requires novel LHP1-interacting domain and ATPase activity of the ATRX SNF2 helicase domain. Taken together, these results suggest that distinct evolutionary pathways led to the interaction between ATRX and HP1 in mammals and its counterpart LHP1 in plants, resulting in distinct modes of transcriptional regulation. |
| Databáze: | OpenAIRE |
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