A new hexapeptide from the leader peptide of rMnSOD enters cells through the oestrogen receptor to deliver therapeutic molecules
| Autor: | Antonella Borrelli, Piero Pucci, Andrea Carpentieri, F. Tuccillo, Marina Sanseverino, Viviana De Luca, P. Bonelli, Leila Birolo, Antonietta Schiattarella, Jean Rommelaere, Alessandra Pica, Enrico Gori, Roberto Mancini, Maria Laura Pollio, Clemente Capasso, Maria Grazia Ruggiero, Aldo Mancini |
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| Přispěvatelé: | Borrelli, A., Schiattarella, A., Mancini, R., Pica, Alessandra, Pollio, MARIA LAURA, Ruggiero, MARIA GRAZIA, Bonelli, P., De Luca, V., Tuccillo, F. M., Capasso, Clemente, Gori, E., Sanseverino, M., Carpentieri, Andrea, Birolo, Leila, Pucci, Pietro, Rommelaere, J., Mancini, A. |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell Survival media_common.quotation_subject leader peptide Antineoplastic Agents Protein Sorting Signals Pharmacology Endoplasmic Reticulum Bioinformatics Article law.invention Inhibitory Concentration 50 03 medical and health sciences 0302 clinical medicine Therapeutic index law Cell Line Tumor medicine Humans Cytotoxic T cell Amino Acid Sequence Internalization Peptide sequence Fluorescent Dyes media_common Cisplatin chemistry.chemical_classification Drug Carriers Multidisciplinary Superoxide Dismutase business.industry Peptide Fragments Recombinant Proteins Amino acid 030104 developmental biology Receptors Estrogen chemistry Cell culture 030220 oncology & carcinogenesis Recombinant DNA Fluorescein Drug Screening Assays Antitumor business medicine.drug |
| Zdroj: | Scientific reports (Nature Publishing Group) 6 (2016). doi:10.1038/srep18691 info:cnr-pdr/source/autori:Borrelli, Antonella; Schiattarella, Antonietta; Mancini, Roberto; Pica, Alessandra; Pollio, Maria Laura; Ruggiero, Maria Grazia; Bonelli, Patrizia; De Luca, Viviana; Tuccillo, Franca Maria; Capasso, Clemente; Gori, Enrico; Sanseverino, Marina; Carpentieri, Andrea; Birolo, Leila; Pucci, Piero; Rommelaere, Jean; Mancini, Aldo/titolo:A new hexapeptide from the leader peptide of rMnSOD enters cells through the oestrogen receptor to deliver therapeutic molecules/doi:10.1038%2Fsrep18691/rivista:Scientific reports (Nature Publishing Group)/anno:2016/pagina_da:/pagina_a:/intervallo_pagine:/volume:6 Scientific Reports |
| DOI: | 10.1038/srep18691 |
| Popis: | A 24-amino acid leader peptide of a new human recombinant manganese superoxide dismutase can enter cells and carry molecules. Here, we demonstrated that six of the 24 amino acids penetrate cells through a particular gate represented by a specific amino acid sequence of the oestrogen receptor (ER). We analysed the internalization of the synthetic hexapeptide and the cytotoxic activity of the hexapeptide conjugated to cisplatin on a cell line panel. In most cell lines, the hexapeptide delivered an amount of cisplatin that was 2 to 8 times greater than that released by cisplatin when the drug was used alone. This increased delivery increases the therapeutic index of cisplatin and reduces side effects caused by a high dosage or long-term treatment times. We may consider this hexapeptide a new molecular carrier to deliver molecules with therapeutic activity into ER+ cells for diagnostic purposes and clinical or immune therapy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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