Microbial Glycosylation of Macrolide Antibiotics by Streptomyces hygroscopicus ATCC 31080 and Distribution of a Macrolide Glycosyl Transferasein Several Streptomyces Strains
| Autor: | Shigeo Morimoto, Joji Sasaki, Kazutoshi Mizoue, Sadafumi Omura |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Glycosylation
medicine.drug_class Stereochemistry Microbial Sensitivity Tests Tylosin Streptomyces Macrolide Antibiotics chemistry.chemical_compound Transferases Drug Discovery medicine Antibacterial agent Pharmacology Oleandomycin biology Desosamine Streptomycetaceae biology.organism_classification Anti-Bacterial Agents Erythromycin carbohydrates (lipids) chemistry Biochemistry Streptomyces hygroscopicus medicine.drug |
| Zdroj: | The Journal of Antibiotics. 49:1110-1118 |
| ISSN: | 1881-1469 0021-8820 |
| Popis: | In the course of our microbial transformation study on erythromycin derivatives, Streptomyces hygroscopicus ATCC 31080, which produces a poly ether antibiotic carriomycin, was found to transform erythromycin derivatives to their inactivated derivatives. The structures of inactivated derivatives prepared by enzyme reaction using the cell extract, UDP-glucose (or UDP-galactose) and Mg2+ (or Mn2+) were elucidated on the basis of analysis of their spectral data to be the compounds glycosylated at C-2' of a desosamine moiety, indicating that the enzyme is a macrolide glycosyl transferase (MGT). The MGT activity of cell extract from S. antibiotieus ATCC 11891, a producing organism of oleandomycin, could be distinguished from that of ATCC 31080, based on the ability to glycosylate tylosin. We examined 32 actinomycete strains producing such polyketides as macrolide and polyether antibiotics, and found that 15 strains of Streptomyces have macrolide glycosyl transferase activity. It suggests that the MGTs have been distributed among at least polyketide producing Streptomyces strains. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|