Enhancement of Viable Adipose-Derived Stem Cells in Lipoaspirate by Buffering Tumescent with Sodium Bicarbonate
| Autor: | Joshua J. Goldman, Shelley J. Williams, Ashish Francis, Xin-Hua Fang, Wei Z. Wang, Richard C. Baynosa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Necrosis Lidocaine medicine.medical_treatment Urology lcsh:Surgery Adipose tissue 030230 surgery Experimental 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Graft take medicine Sodium bicarbonate business.industry lcsh:RD1-811 Plastic surgery chemistry 030220 oncology & carcinogenesis Liposuction Surgery medicine.symptom Stem cell business medicine.drug |
| Zdroj: | Plastic and Reconstructive Surgery, Global Open, Vol 7, Iss 3, p e2138 (2019) BASE-Bielefeld Academic Search Engine Plastic and Reconstructive Surgery Global Open |
| ISSN: | 2169-7574 |
| DOI: | 10.1097/GOX.0000000000002138 |
| Popis: | Background:. Fat grafting is a growing field within plastic surgery. Adipose-derived stem cells (ASCs) and stromal vascular fracture (SVF) may have a role in fat graft survival. Our group previously demonstrated a detrimental effect on ASC survival by the lidocaine used in tumescent solution. Sodium bicarbonate (SB) buffers the acidity of lidocaine. The purpose of this study was to determine whether SB buffering is a practical method to reduce ASC and SVF apoptosis and necrosis seen with common lidocaine-containing tumescent solution. Methods:. Human patients undergoing bilateral liposuction for any indication were included in this study. An internally controlled, split-body design was utilized. Tumescent liposuction on one side of the body was conducted with tumescent containing lidocaine. On the opposite side, liposuction was conducted by adding SB to the tumescent. Tumescent solution and lipoaspirate pH were measured. Lipoaspirate from each side was processed for SVF isolation and ASC culture. The number of viable ASCs was counted and SVF apoptosis/necrosis was examined. Results:. The pH of the SB-buffered tumescent was significantly higher than that of the standard tumescent, an effect also seen in the lipoaspirate. Adipose-derived stem cell survival in the SB-buffered lipoaspirate was approximately 53% higher. However, there was no significant difference in SVF apoptosis and necrosis between the groups. Conclusions:. The acidic standard tumescent solution commonly used in liposuction diminishes ASC viability from lipoaspirates. Sodium bicarbonate buffering tumescent solution can enhance ASC viability, but does not affect SVF apoptosis and necrosis. We recommend buffering tumescent with SB to potentially improve fat graft take. Our findings advocate for further research investigating mechanisms and optimal harvest techniques that maximize SVF/ASC survival and the clinical effect on overall fat graft viability. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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