Estimation of the effectiveness ratio (α/β) for resistant cancer cells in U87MG human glioblastoma
| Autor: | Nallely Jiménez-Mancilla, Erika Azorín-Vega, Eugenio Torres-García, Perla Marmolejo-León, Eleni Mitsoura, Benjamín Pineda, Héctor Mendoza-Nava |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell Survival Cellular differentiation Tumor initiation Radiation Tolerance Flow cytometry 03 medical and health sciences 0302 clinical medicine Cancer stem cell Radioresistance Glioma Cell Line Tumor Spheroids Cellular medicine Tumor Microenvironment Humans AC133 Antigen Cell Proliferation Tumor microenvironment Radiation medicine.diagnostic_test Cell growth Chemistry Brain Neoplasms Cell Differentiation Dose-Response Relationship Radiation medicine.disease 030104 developmental biology 030220 oncology & carcinogenesis Cancer research Neoplastic Stem Cells Glioblastoma |
| Zdroj: | Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine. 141 |
| ISSN: | 1872-9800 |
| Popis: | Glioblastoma contains self-renewing, tumorigenic cancer stem-like cells that contribute to tumor initiation and therapeutic resistance. The aim of this research was to estimate and compare the effectiveness ratio (α/β) of stem-like cells and differentiated glioma cells derived from the U87MG glioblastoma cell line. Cell survival experiments were obtained in a dose range of 0-20 Gy (13.52 ± 0.09 Gy/h) as a hyperfractionationated accelerated radiotherapy scheme. Biochemical characterization of the post-irradiated cells was performed by flow cytometry analysis and the percentage of stem-like cells that resisted irradiation was determined by the CD133 expression. Results showed that U87MG stem-like cells are highly proliferative and more radioresistant than the U87MG adherent group (with a lesser stem-like character), this in association with the calculated α/β ratio of 17 and 14.1, respectively. |
| Databáze: | OpenAIRE |
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