A comparison of blood and cerebrospinal fluid cytokines (IL-1β, IL-6, IL-18, TNF-α) in neonates with perinatal hypoxia
| Autor: | Filip Čulo, Melanie Ivana Čulo, Marjana Jerković-Raguž, Paško Konjevoda, Darinka Šumanović-Glamuzina |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Interleukin-1beta Encephalopathy Ischemia Neonates cytokines perinatal hypoxia hypoxic-ischemic encephalopathy neurological outcome IL-1β IL-6 IL-18 TNF-α Gastroenterology Cohort Studies 03 medical and health sciences 0302 clinical medicine Cerebrospinal fluid Pregnancy Internal medicine Humans Medicine Prospective Studies Hypoxia Brain Prospective cohort study lcsh:R5-920 Asphyxia Neonatorum Interleukin-6 Tumor Necrosis Factor-alpha business.industry Infant Newborn Interleukin-18 Interleukin General Medicine Hypoxia (medical) medicine.disease Treatment Outcome 030104 developmental biology Hypoxia-Ischemia Brain Immunology Cytokines Female Interleukin 18 Nervous System Diseases medicine.symptom lcsh:Medicine (General) business Meningitis 030217 neurology & neurosurgery Research Article |
| Zdroj: | Bosnian Journal of Basic Medical Sciences, Vol 17, Iss 3 (2017) |
| ISSN: | 1840-4812 1512-8601 |
| Popis: | Perinatal hypoxia-ischemia is a specific and important pathological event in neonatal care practice. The data on relationship between the concentrations of cytokines in blood and cerebrospinal fluid (CSF) and perinatal brain injury are scarce. The aim of this study is to evaluate changes in interleukin (IL-1β, IL-6, and IL-18) and tumor necrosis factor alpha (TNF-α) levels in newborns with perinatal hypoxia (PNH). CSF and serum samples of 35 term and near-term (35-40 weeks) newborns with PNH, at the age of 3-96 hours, were analyzed using enzyme-linked immunosorbent assay. Control group consisted of 25 non-asphyxic/non-hypoxic infants of the same age sampled for clinically suspected perinatal meningitis, but proven negative and healthy otherwise. The cytokine values in CSF and serum samples were determined in relation to initial hypoxic-ischemic encephalopathy (HIE) staged according the Sarnat/Sarnat method, and compared with neurological outcome at 12 months of age estimated using Amiel-Tison procedure. The concentrations of IL-6 and TNF-α in serum of PNH patients were significantly higher compared to control group (p = 0.0407 and p = 0.023, respectively). No significant difference between average values of cytokines in relation to the stage of HIE was observed. Significantly higher levels of IL-6 and IL-18 corresponded to a mildly abnormal neurological outcome, while higher levels of IL-6 and TNF-α corresponded to a severely abnormal neurological outcome, at 12 months of age. Elevated serum levels of IL-6 and TNF-α better corresponded with hypoxia/ischemia compared to CSF values, within 96 hours of birth. Also, higher serum levels of IL-6, TNF-α, and IL-18 corresponded better with abnormal neurological outcome at 12 months of age, compared to CSF values. |
| Databáze: | OpenAIRE |
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