Everything you ever wanted to know about PKA regulation and its involvement in mammalian sperm capacitation
| Autor: | Iñaki Gentile, Cintia Stival, Carla Ritagliati, Guillermina Maria Luque, Dario Krapf, Carolina Baro Graf, Mariano G. Buffone |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male 030209 endocrinology & metabolism Context (language use) Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Capacitation Animals Humans Cyclic adenosine monophosphate Protein kinase A Molecular Biology Mammals Mammalian sperm Chemistry Acrosome Reaction Sperm Cyclic AMP-Dependent Protein Kinases Spermatozoa Cell biology 030104 developmental biology Protein Processing Post-Translational Sperm Capacitation Signal Transduction |
| Zdroj: | Molecular and cellular endocrinology. 518 |
| ISSN: | 1872-8057 |
| Popis: | The 3', 5'-cyclic adenosine monophosphate (cAMP) dependent protein kinase (PKA) is a tetrameric holoenzyme comprising a set of two regulatory subunits (PKA-R) and two catalytic (PKA-C) subunits. The PKA-R subunits act as sensors of cAMP and allow PKA-C activity. One of the first signaling events observed during mammalian sperm capacitation is PKA activation. Thus, understanding how PKA activity is restricted in space and time is crucial to decipher the critical steps of sperm capacitation. It is widely accepted that PKA specificity depends on several levels of regulation. Anchoring proteins play a pivotal role in achieving proper localization signaling, subcellular targeting and cAMP microdomains. These multi-factorial regulation steps are necessary for a precise spatio-temporal activation of PKA. Here we discuss recent understanding of regulatory mechanisms of PKA in mammalian sperm, such as post-translational modifications, in the context of its role as the master orchestrator of molecular events conducive to capacitation. |
| Databáze: | OpenAIRE |
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