Formation and metabolism of [14C]dopamine 3-O-sulfate in dog, rat and guinea pig
| Autor: | Ilmar Merits |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Male
medicine.medical_specialty Dopamine Guinea Pigs Deamination Urine In Vitro Techniques Pharmacology Biochemistry Guinea pig Dogs Sulfate conjugate Species Specificity Oral administration Internal medicine Intestine Small medicine Animals biology Metabolism In vitro Rats Endocrinology biology.protein Female medicine.drug |
| Zdroj: | Biochemical Pharmacology. 25:829-833 |
| ISSN: | 0006-2952 |
| Popis: | Oral administration of [14C]dopamine to dogs resulted in urinary excretion of predominantly [14C]dopamine 3-O-sulfate, while after intravenous administration the labeled drug was metabolized largely by O-methylation and deamination pathways. Experiments in vitro pinpointed the small intestinal wall of the dog as the site of dopamine sulfate conjugate formation. The small intestines of rat and guinea pig lack this sulfating ability. When trace amounts of [14C]dopamine 3-O-sulfate were administered to dog, rat and guinea pig, the compound turned out not to be metabolically inert. In the guinea pig, [14C]dopamine 3-O-sulfate was almost completely desulfated and metabolized according to the pattern characteristic to orally administered dopamine in this animal species. In rat, about 40 per cent of the administered [14C]dopamine 3-O-sulfate (19.1 μg/kg) was excreted in urine unchanged, whereas a smaller dose (7.4 μg/kg) was totally metabolized according to the pattern characteristic to rat. In dog urine, more than 80 per cent of the radioactive dose (2.5 μg/kg) emerged in urine as unchanged [14C]dopamine 3-O-sulfate, the normal metabolism end product of dopamine in dog. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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