Rit contributes to neurite outgrowth triggered by the alpha subunit of Go
| Autor: | Seung Hyun Kim, Soyeon Kim, Sung Ho Ghil |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Neurite
G protein Cellular differentiation GTP-Binding Protein alpha Subunits Green Fluorescent Proteins macromolecular substances Biology Transfection Cell Line Mice Heterotrimeric G protein Two-Hybrid System Techniques Neurites Animals Humans Immunoprecipitation Small GTPase Phosphorylation Extracellular Signal-Regulated MAP Kinases G alpha subunit General Neuroscience Cell Differentiation Cell biology ras Proteins Signal transduction Neuroscience Signal Transduction |
| Zdroj: | Neuroreport. 19(5) |
| ISSN: | 0959-4965 |
| Popis: | Heterotrimeric GTP-binding protein transduce signals initiated by a variety of hormones and neurotransmitters. Go, a member of the Go/Gi family, is the most abundant heterotrimeric GTP-binding protein in nervous tissues and has been implicated in neuronal differentiation. The mechanism by which Go modulates neuronal differentiation has not been, however, fully elucidated. Here, we identified small GTPase Rit as an interacting partner of the alpha-subunit of Go (Goalpha). The biochemical characterizations of Goalpha::Rit interaction revealed that Rit is a candidate downstream effector for Goalpha. Furthermore, dominant negative Rit inhibited Goalpha-induced neurite outgrowth and Erk phosphorylation in Neuro2a cells. These results suggest that Rit may be involved in the signaling pathway for Goalpha-mediated neuronal differentiation. |
| Databáze: | OpenAIRE |
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