Downregulation of parathyroid hormone receptor gene expression and osteoblastic dysfunction associated with skeletal resistance to parathyroid hormone in a rat model of renal failure with low turnover bone
| Autor: | Hideyuki Yamato, Kiyoshi Kurokawa, Masafumi Fukagawa, Tomoko Nii-Kono, Yoshiko Iwasaki-Ishizuka |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Bone disease Injections Subcutaneous Drug Resistance Parathyroid hormone Polymerase Chain Reaction Bone and Bones Rats Sprague-Dawley Downregulation and upregulation Internal medicine Animals Medicine Infusions Intravenous Receptor DNA Primers Receptor Parathyroid Hormone Type 1 Transplantation Osteoblasts Base Sequence business.industry Parathyroid hormone receptor Osteoblast Alkaline Phosphatase medicine.disease Actins Rats Disease Models Animal Thyroxine Endocrinology medicine.anatomical_structure Parathyroid Hormone Nephrology Kidney Failure Chronic Alkaline phosphatase Secondary hyperparathyroidism business |
| Zdroj: | Nephrology Dialysis Transplantation. 20:1904-1911 |
| ISSN: | 1460-2385 0931-0509 |
| DOI: | 10.1093/ndt/gfh876 |
| Popis: | Background. Adynamic bone disease (ABD), which is characterized by reduced bone formation and resorption, has become an increasingly common manifestation of bone abnormalities in patients with end-stage renal failure. It has been recognized that skeletal resistance to parathyroid hormone (PTH) underlies the pathogenesis of ABD; however, the mechanisms of such resistance remain unclear. Methods. We established a rat model simulating ABD under chronic renal failure conditions by thyroparathyroidectomy and partial nephrectomy (TPTxNx). TPTx-Nx rats were infused subcutaneously with a physiological dose of PTH. We analysed bone histomorphometric parameters and demonstrated gene expression using semi-quantitative reverse transcription–polymerase chain reaction. Results. Reduced bone formation was observed in this model, simulating ABD. The reduction was dependent on the degree of renal dysfunction. Bone formation rate was 6.4±2.7mm 3 /m 2 /year in TPTx5/6Nx rats and 22.7±7.2mm 3 /m 2 /year in TPTx rats (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|