A Novel High-Resolution Melt PCR Assay Discriminates Anaplasma phagocytophilum and 'Candidatus Neoehrlichia mikurensis'
| Autor: | Stefanie Beck, Mareen Kohn, Janina Demeler, Georg von Samson-Himmelstjerna, Stefan Pachnicke, Denny Maaz, Franz-Rainer Matuschka, Dania Richter, Barbara Kohn, Philipp Olias, Cécile Schreiber, Jürgen Krücken, Eberhard Schein, Klemens Krieger |
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| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
Ixodes ricinus Ixodes hexagonus animal diseases Rodentia Polymerase Chain Reaction Microbiology Dermacentor reticulatus parasitic diseases Animals Dermacentor Bacteriological Techniques Ixodes biology biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Anaplasma phagocytophilum Virology Anaplasmataceae Molecular Diagnostic Techniques Candidatus bacteria Parasitology |
| Zdroj: | Journal of Clinical Microbiology. 51:1958-1961 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.00284-13 |
| Popis: | “ Candidatus Neoehrlichia mikurensis” ( Anaplasmataceae ) is an emerging pathogen transmitted by Ixodes ticks. Conventional PCR and the newly developed high-resolution melt PCR were used to detect and discriminate “ Candidatus Neoehrlichia mikurensis” and Anaplasma phagocytophilum . Both bacterial species were frequently found in Ixodes ricinus and Ixodes hexagonus but virtually absent from Dermacentor reticulatus . In rodents, “ Candidatus N. mikurensis” was significantly more prevalent than A. phagocytophilum , whereas in cats, only A. phagocytophilum was found. |
| Databáze: | OpenAIRE |
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