A Lactobacillus acidophilus Strain of Human Gastrointestinal Microbiota Origin Elicits Killing of Enterovirulent Salmonella enterica Serovar Typhimurium by Triggering Lethal Bacterial Membrane Damage
| Autor: | Marie-Hélène Coconnier-Polter, Alain L. Servin, Vanessa Liévin-Le Moal |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Lipopolysaccharides
Salmonella typhimurium Salmonella Cell Membrane Permeability Lipopolysaccharide Biology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Adenosine Triphosphate Bacteriolysis Lactobacillus acidophilus Antibiosis medicine Humans Pathogen Ecology Cell Membrane Gene Expression Regulation Bacterial Physiology and Biotechnology biology.organism_classification Enterobacteriaceae Gastrointestinal Tract chemistry Lytic cycle Salmonella enterica Culture Media Conditioned Bacteria Signal Transduction Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 71:6115-6120 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.71.10.6115-6120.2005 |
| Popis: | The human gastrointestinal microbiota produces antagonistic activities against gastrointestinal bacterial pathogens. We undertook a study to investigate the mechanism(s) by which a Lactobacillus acidophilus strain of human microbiota origin antagonizes the gram-negative enteroinvasive pathogen Salmonella enterica serovar Typhimurium. We showed that the cell-free culture supernatant of L. acidophilus strain LB (LB-CFCS) induced the following effects in S. enterica SL1344: (i) a decrease in intracellular ATP that paralleled bacterial death, (ii) the release of lipopolysaccharide, (iii) permeabilization of the bacterial membrane, and (iv) an increase in the sensitivity of Salmonella to the lytic action of sodium dodecyl sulfate. Finally, we showed using two mutant strains of Salmonella , PhoP MS7953s and PmrA JKS1170, that the two-component regulatory systems PhoP-PhoQ and PmrA-PmrB that regulate the mechanisms of resistance to antibacterial agents in Salmonella did not influence the anti- Salmonella effect of LB-CFCS. |
| Databáze: | OpenAIRE |
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