LC-ESI-MS/MS Analysis of Sulfolipids and Galactolipids in Green and Red Lettuce (Lactuca sativa L.) as Influenced by Sulfur Nutrition
Autor: | Tania T. Körber, Tobias Sitz, Muna A. Abdalla, Karl H. Mühling, Sascha Rohn |
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Rok vydání: | 2023 |
Předmět: |
Inorganic Chemistry
glycolipids oxidized SQDG S-limiting condition Organic Chemistry General Medicine Physical and Theoretical Chemistry 500 Naturwissenschaften und Mathematik::540 Chemie::540 Chemie und zugeordnete Wissenschaften fatty acids Molecular Biology Spectroscopy Catalysis Computer Science Applications |
Zdroj: | International Journal of Molecular Sciences Volume 24 Issue 4 Pages: 3728 |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms24043728 |
Popis: | Sulfur (S) deprivation leads to abiotic stress in plants. This can have a significant impact on membrane lipids, illustrated by a change in either the lipid class and/or the fatty acid distribution. Three different levels of S (deprivation, adequate, and excess) in the form of potassium sulfate were used to identify individual thylakoid membrane lipids, which might act as markers in S nutrition (especially under stress conditions). The thylakoid membrane consists of the three glycolipid classes: monogalactosyl- (MGDG), digalactosyl- (DGDG), and sulfoquinovosyl diacylglycerols (SQDG). All of them have two fatty acids linked, differing in chain length and degree of saturation. LC-ESI-MS/MS served as a powerful method to identify trends in the change in individual lipids and to understand strategies of the plant responding to stress. Being a good model plant, but also one of the most important fresh-cut vegetables in the world, lettuce (Lactuca sativa L.) has already been shown to respond significantly to different states of sulfur supply. The results showed a transformation of the glycolipids in lettuce plants and trends towards a higher degree of saturation of the lipids and an increased level of oxidized SQDG under S-limiting conditions. Changes in individual MGDG, DGDG, and oxidized SQDG were associated to S-related stress for the first time. Promisingly, oxidized SQDG might even serve as markers for further abiotic stress factors. |
Databáze: | OpenAIRE |
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