Probing the Eumelanin-Silica Interface in Chemically Engineered Bulk Hybrid Nanoparticles for Targeted Subcellular Antioxidant Protection
| Autor: | Giuseppe Vitiello, Brigida Silvestri, Mariagrazia Iacomino, Alessandro Pezzella, Simona Paladino, Gerardino D'Errico, Giuseppina Luciani, M. Federica Caso, Vincenzo Calcagno, Marco d'Ischia, Aniello Costantini, Maria Gallo, Silvia Parisi |
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| Přispěvatelé: | Silvestri, Brigida, Vitiello, Giuseppe, Luciani, Giuseppina, Calcagno, Vincenzo, Costantini, Aniello, Gallo, Maria, Parisi, Silvia, Paladino, Simona, Iacomino, Mariagrazia, D'Errico, Gerardino, Caso, M. Federica, Pezzella, Alessandro, D'Ischia, Marco |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Materials science
Indoles antioxidant hybrid nanomaterial Nanoparticle 02 engineering and technology engineering.material 010402 general chemistry 01 natural sciences HeLa eumelanin chemistry.chemical_compound cytoprotection Cell Line Tumor Humans General Materials Science Hydrogen peroxide Melanins oxidative stre biology DHICA Settore CHIM/06 - Chimica Organica 021001 nanoscience & nanotechnology biology.organism_classification Silicon Dioxide 0104 chemical sciences chemistry Biochemistry Polymerization Covalent bond lysosomal localization silica nanoparticle engineering Biophysics Nanoparticles Biopolymer Materials Science (all) 0210 nano-technology Conjugate |
| Popis: | We disclose herein the first example of stable monodispersed hybrid nanoparticles (termed MelaSil−NPs) made up of eumelanin biopolymer intimately integrated into a silica nanoscaffold matrix and endowed with high antioxidant and cytoprotective effects associated with a specific subcellular localization. MelaSil−NPs have been fabricated by an optimized sol−gel methodology involving ammonia-induced oxidative polymerization of a covalent conjugate of the eumelanin building block 5,6-dihydroxyindole-2-carboxylic acid (DHICA) with 3-aminopropyltriethoxysilanes (APTS). They displayed a round-shaped (ca. 50−80 nm) morphology, exhibited the typical electron paramagnetic resonance signal of eumelanin biopolymers, and proved effective in promoting decomposition of hydrogen peroxide under physiologically relevant conditions. When administered to human ovarian cancer cells (A2780) or cervical cancer cells (HeLa), MelaSil−NPs were rapidly internalized and colocalized with lysosomes and exerted efficient protecting effects against hydrogen peroxide-induced oxidative stress and cytotoxicity. We disclose herein the first example of stable monodispersed hybrid nanoparticles (termed MelaSil-NPs) made up of eumelanin biopolymer intimately integrated into a silica nanoscaffold matrix and endowed with high antioxidant and cytoprotective effects associated with a specific subcellular localization. MelaSil-NPs have been fabricated by an optimized sol-gel methodology involving ammonia-induced oxidative polymerization of a covalent conjugate of the eumelanin building block 5,6-dihydroxyindole-2-carboxylic acid (DHICA) with 3-aminopropyltriethoxysilanes (APTS). They displayed a round-shaped (ca. 50-80 nm) morphology, exhibited the typical electron paramagnetic resonance signal of eumelanin biopolymers, and proved effective in promoting decomposition of hydrogen peroxide under physiologically relevant conditions. When administered to human ovarian cancer cells (A2780) or cervical cancer cells (HeLa), MelaSil-NPs were rapidly internalized and colocalized with lysosomes and exerted efficient protecting effects against hydrogen peroxide-induced oxidative stress and cytotoxicity. |
| Databáze: | OpenAIRE |
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