A novel selective medium for the detection of methicillin-resistant Staphylococcus aureus enabling result reporting in under 24h
| Autor: | Marilyn Ford, John D. Perry, S. Morgan, K.E. Orr, C Gurran, M.G. Holliday |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Microbiology (medical)
Staphylococcus aureus Time Factors Micrococcaceae Aztreonam medicine.disease_cause Microbiology chemistry.chemical_compound Humans Medicine Phenol red biology business.industry General Medicine Staphylococcal Infections biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Methicillin-resistant Staphylococcus aureus Bacterial Typing Techniques Ciprofloxacin Infectious Diseases chemistry Colistin Methicillin Resistance Mannitol business medicine.drug |
| Zdroj: | Journal of Hospital Infection. 52:148-151 |
| ISSN: | 0195-6701 |
| DOI: | 10.1053/jhin.2002.1260 |
| Popis: | A simple selective broth was devised to indicate the presence of methicillin resistant Staphylococcus aureus (MRSA) in clinical samples. The broth comprised nutrient broth supplemented with sodium chloride, ciprofloxacin, colistin and aztreonam as selective agents and also mannitol, trehalose and phenol red as an indicator system. In a preliminary study using 228 clinical samples this selective mannitol broth (SMB) proved to be more sensitive than other selective agars for detection of MRSA within 24 h. In an extended study of a further 1124 clinical samples from 470 randomly selected patients, SMB detected 85.1% of MRSA strains present with a specificity of 43.6%. We conclude that SMB offers a convenient, inexpensive and sensitive method for high-throughput screening for MRSA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect