In vitro effects of 1α,25(OH)2D3-glycosides from Solbone A (Solanum glaucophyllum leaves extract; Herbonis AG) compared to synthetic 1α,25(OH)2D3 on myogenesis
| Autor: | Pablo De Genaro, Valeria Gili, Ana Carolina Ronda, Ricardo Boland, Verónica González Pardo, Ana Russo de Boland, Heini Bachmann |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Clinical Biochemistry 030209 endocrinology & metabolism Biology Biochemistry Ciencias Biológicas 03 medical and health sciences 0302 clinical medicine Endocrinology Myosin Myocyte C2C12 cells Synthetic 1a 25(OH)(2)D(3) Solbone A Molecular Biology Myogenin Solanum glaucophyllum Pharmacology chemistry.chemical_classification Myogenesis Organic Chemistry Glycoside Bioquímica y Biología Molecular biology.organism_classification Tropomyosin 030104 developmental biology chemistry Differentiation C2C12 CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | Steroids. 109:7-15 |
| ISSN: | 0039-128X |
| DOI: | 10.1016/j.steroids.2016.03.002 |
| Popis: | The presence of glycoside derivatives of 1α,25(OH)2D3 endows plants to gradual release of the free bioactive form of 1α,25(OH)2D3 from its glycoconjugates by endogenous animal tissue glycosidases. This results in increased half-life of the hormone in blood when purified plant fractions are administered for therapeutic purposes. In this work, we evaluated the role 1α,25(OH)2D3-glycosides enriched natural product (Solbone A) from Solanum glaucophyllum leaf extract compared with synthetic 1α,25(OH)2D3 on myogenic differentiation in C2C12 myoblasts. For these, differentiation markers and myogenic parameters were studied in C2C12 myoblasts. Results showed that Solbone A, likewise the synthetic hormone, increased creatine kinase activity at day 2 after differentiation induction (60%, p |
| Databáze: | OpenAIRE |
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