Enzymatic and acidic degradation effect on intracellular polysaccharide of Flammulina velutipes SF-08
| Autor: | Zhao Ma, Fangyuan Cui, Le Jia, Mengshi Jia, Shouhua Jia, Jianjun Zhang, Xia Gao, Chen Zhang |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Antioxidant Rhamnose medicine.medical_treatment Polysaccharide Biochemistry Antioxidants Catalysis Superoxide dismutase chemistry.chemical_compound Mice Structural Biology medicine Monosaccharide Animals Molecular Biology Flammulina chemistry.chemical_classification Glutathione Peroxidase biology Dose-Response Relationship Drug Superoxide Dismutase Glutathione peroxidase Hydrolysis Age Factors Fungal Polysaccharides General Medicine biology.organism_classification Catalase Enzyme chemistry biology.protein Lipid Peroxidation Oxidation-Reduction |
| Zdroj: | International journal of biological macromolecules. 73 |
| ISSN: | 1879-0003 |
| Popis: | The intracellular polysaccharide (IPS) from Flammulina velutipes SF-08 mycelia was isolated and degraded by enzyme and acid. IPS and its derivative were purified by DEAE-52 cellulose chromatography, and five fractions were obtained. The structural features and antioxidant activities in vitro of the isolated fractions were evaluated. On the basis of chemical composition and antioxidant ability analyses, rhamnose as the main monosaccharide might contribute to the strongest antioxidant capacity. The in vivo results showed that IPS significantly enhanced the activities of anti-aging enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and reduced the content of lipid peroxidantion (LPO). These results suggested that IPS should be a potent natural polymer and can be developed to be novel functional food. |
| Databáze: | OpenAIRE |
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