Antibiotic release and antibacterial efficacy in cement spacers and cement beads impregnated with different techniques: In vitro study
| Autor: | Cüneyt Özakin, Namık Şahin, Tufan Kaleli, Teoman Atici, Sinan Çavun |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
musculoskeletal diseases
Staphylococcus aureus Prosthesis-Related Infections food.ingredient medicine.drug_class Arthroplasty Replacement Hip Antibiotics In Vitro Techniques Bead 03 medical and health sciences Drug Delivery Systems 0302 clinical medicine food Vancomycin medicine Humans Agar Orthopedics and Sports Medicine 030212 general & internal medicine Antibiotic release Cement 030222 orthopedics Chromatography Cement spacers business.industry Rehabilitation Bone Cements technology industry and agriculture Anti-Bacterial Agents visual_art visual_art.visual_art_medium Surgery Gentamicin Gentamicins business medicine.drug |
| Zdroj: | Joint Diseases and Related Surgery. 29:71-78 |
| ISSN: | 1309-0313 1305-8282 |
| DOI: | 10.5606/ehc.2018.59021 |
| Popis: | This study aims to compare the antibiotic release and biological effectiveness of bead type and articulating spacers of different cement types with antibiotics added at alternative phases of cement preparation.Four gram vancomycin was added into two types of antibiotic-free cement (Simplex®, Biomet®) with similar viscosity and also gentamycin-containing cement (Refobacin®). Prepared specimens were used to create cement beads and articulating hip spacers, making a total of six different groups. Two alternative groups were formed by adding the Vancomycin while the cement was in dough phase. Antibiotic release and biological activity were evaluated with immunoassay techniques and agar-disk diffusion methods.All groups showed initial antibiotics surge in the first week, which was 2 to 4 times more evident in the beads group. Antibiotic release and change in release rate were significantly different between Simplex-alternative and Simplex, Biomet, Refobacin-beads, and between Biomet-spacer and Refobacin-beads groups (p0.05). Elution of antibiotics was not different between mobile spacers prepared with conventional or alternative methods (p0.05). Biomet cement showed larger diffusion inhibition zone in agar. There was no difference between biological activity of the bead and mobile designs of the Biomet brand (p0.05). Inhibition zone analyses of agar and disk diffusion tests revealed significant differences between several groups (p0.05).Cement beads provide superior antibiotic release regardless of cement type or preparation method. Simplex P® cement has lower anti-bacterial efficiency than Biomet®. Different methods for cement and antibiotics mixing and addition of extra vancomycin into the commercially drug loaded cement do not have any effect on the results. |
| Databáze: | OpenAIRE |
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