Two nematicidal furocoumarins from Ficus carica L. leaves and their physiological effects on pine wood nematode (Bursaphelenchus xylophilus)
| Autor: | Daosen Guo, Guicai Du, Hongwei He, Hongkai Xu, Ronggui Li, Qunqun Guo |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
Tylenchida Nematoda Ethyl acetate Ficus Bursaphelenchus xylophilus Plant Science 01 natural sciences Biochemistry Bergapten Analytical Chemistry chemistry.chemical_compound Cellulase Furocoumarins Botany Animals Amylase Psoralen biology Antinematodal Agents Organic Chemistry Ficusin biology.organism_classification 0104 chemical sciences Plant Leaves 010404 medicinal & biomolecular chemistry chemistry Amylases biology.protein 5-Methoxypsoralen Methoxsalen Cholinesterase Inhibitors Carica 010606 plant biology & botany |
| Zdroj: | Natural product research. 30(17) |
| ISSN: | 1478-6427 |
| Popis: | The ethanol extract of the Ficus carica L. leaves was tested to show strong nematicidal activity against pine wood nematode (PWN), Bursaphelenchus xylophilus, causing 90.93% corrected mortality within 72 h at 1.0 mg/mL. From the ethyl acetate soluble fraction of the F. carica L. leaves extract, the main nematicidal constituents were obtained by bioassay-guided isolation and identified as linear furocoumarins bergapten (1) and psoralen (2) by mass and NMR spectral data analysis. Bergapten and psoralen had significant nematicidal activity against PWN with the LC50 values of 97.08 aKSnd 115.03 μg/mL within 72 h, respectively. The two furocoumarins could inhibit the activities of amylase, cellulase and acetylcholinesterase (AchE) from PWN. The morphologies of PWNs changed much after they were treated by bergapten and psoralen. The physiological effects of bergapten and psoralen on PWN might provide helpful clues to elucidate their nematicidal mechanisms. |
| Databáze: | OpenAIRE |
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