Chl1 and Ctf4 are required for damage-induced recombinations
| Autor: | Mong Sing Lai, Masayuki Seki, Ayako Ui, Takemi Enomoto, Hideaki Ogiwara |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Recombination
Genetic Saccharomyces cerevisiae Proteins DNA Repair Chromosomal Proteins Non-Histone DNA repair fungi Biophysics Sister chromatid exchange Cell Biology Biology DNA repair protein XRCC4 Biochemistry Molecular biology Genomic Instability DNA-Binding Proteins Establishment of sister chromatid cohesion Non-homologous end joining Homology directed repair Postreplication repair Sister chromatids DNA Fungal Sister Chromatid Exchange Molecular Biology DNA Damage |
| Zdroj: | Biochemical and Biophysical Research Communications. 354:222-226 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2006.12.185 |
| Popis: | Deletion mutants of CHL1 or CTF4, which are required for sister chromatid cohesion, showed higher sensitivity to the DNA damaging agents methyl methanesulfonate (MMS), hydroxyurea (HU), phleomycin, and camptothecin, similar to the phenotype of mutants of RAD52, which is essential for recombination repair. The levels of Chl1 and Ctf4 associated with chromatin increased considerably after exposure of the cells to MMS and phleomycin. Although the activation of DNA damage checkpoint did not affected in chl1 and ctf4 mutants, the repair of damaged chromosome was inefficient, suggesting that Chl1 and Ctf4 act in DNA repair. In addition, MMS-induced sister chromatid recombination in haploid cells, and, more importantly, MMS-induced recombination between homologous chromosomes in diploid cells were impaired in these mutants. Our results suggest that Chl1 and Ctf4 are directly involved in homologous recombination repair rather than acting indirectly via the establishment of sister chromatid cohesion. |
| Databáze: | OpenAIRE |
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