Production of WTC.ZI-zi Rat Congenic Strain and Its Pathological and Genetic Analyses
| Autor: | Takashi Kuramoto, Akira Kondo, Kazuo Nakajima, Ken-ichi Yamasaki, Masao Yamada, Tadao Serikawa |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Central Nervous System
Congenic Cell Communication Biology medicine.disease_cause Rats Mutant Strains General Biochemistry Genetics and Molecular Biology Centimorgan medicine Animals Gene Crosses Genetic Myelin Sheath Genetics Mutation General Veterinary Strain (biology) Chromosome Mapping Chromosome Epistasis Genetic Rats Inbred Strains General Medicine humanities Rats Spinal Cord Backcrossing Microsatellite Animal Science and Zoology Microsatellite Repeats |
| Zdroj: | Experimental Animals. 47:75-81 |
| ISSN: | 1881-7122 1341-1357 |
| DOI: | 10.1538/expanim.47.75 |
| Popis: | A new rat congenic strain, WTC.ZI-zi, was produced after eleven generations of backcrossing between ZI strain as a donor strain and WTC strain as an inbred partner. WTC.ZI-zi/zi homozygous rats generally exhibit more conspicuous body tremor and much earlier occurrence of flaccid paresis than the original ZI strain. The average life span of the congenic strain is approximately nine months, which is also much shorter than that of the original ZI strain. Pathological analysis of the central nervous system of the congenic strain revealed more aggravated vacuolation and hypomyelination than in the original ZI strain. Establishment of the genetic profile with microsatellite markers showed that the congenic strain was genetically almost identical to the WTC strain except for a small chromosome segment bearing the zitter gene. Analysis of markers in this region implied that the length of the donor segment was approximately 13.4 centimorgans which corresponded to 0.65% of the total genome. Thus, these results suggested that expressional alterations of zitter gene were due to replacement of the genetic background from the original ZI strain to the WTC strain. Furthermore, the WTC.ZI-zi congenic strain could provide a refined tool for the analysis of zitter mutation, because the congenic strain has a strict control strain, WTC, and the length of the donor chromosome is genetically defined. |
| Databáze: | OpenAIRE |
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