Dimethyl sulfoxide enhances GLUT4 translocation through a reduction in GLUT4 endocytosis in insulin-stimulated 3T3-L1 adipocytes

Autor: Marion Berenguer, Andrey A. Gurtovenko, Jinzhong Zhang, Yannick Le Marchand-Brustel, Laurène Martinez, Roland Govers, Teresa Gonzalez, Tao Xu, M. Christine Bruce
Přispěvatelé: Centre méditerranéen de médecine moléculaire (C3M), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Northeastern University [Boston], Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Nutrition, obésité et risque thrombotique (NORT), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Center for Rock Instability and Seismicity Research, Northeastern University [Shenyang], Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)
Jazyk: angličtina
Rok vydání: 2011
Předmět:
endocrine system diseases
Glucose uptake
medicine.medical_treatment
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Biochemistry
Mice
0302 clinical medicine
Adipocytes
Insulin
MESH: Animals
0303 health sciences
MESH: Exocytosis
Glucose Transporter Type 4
biology
General Medicine
musculoskeletal system
Endocytosis
Cell biology
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biomolecules [q-bio.BM]
MESH: Glucose
Protein Transport
MESH: Endocytosis
MESH: Molecular Chaperones
hormones
hormone substitutes
and hormone antagonists
MESH: Protein Transport
MESH: Insulin
Exocytosis
03 medical and health sciences
3T3-L1 Cells
medicine
Animals
Dimethyl Sulfoxide
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
MESH: Mice
MESH: Adipocytes
030304 developmental biology
Glucose transporter
MESH: Dimethyl Sulfoxide
nutritional and metabolic diseases
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
MESH: 3T3-L1 Cells
Insulin receptor
Glucose
biology.protein
GLUT1
MESH: Glucose Transporter Type 4
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
030217 neurology & neurosurgery
GLUT4
Molecular Chaperones
Zdroj: Biochimie
Biochimie, Elsevier, 2011, 93 (4), pp.697-709. ⟨10.1016/j.biochi.2010.12.013⟩
Biochimie, Elsevier, 2011, 93 (4), pp.697-709
Biochimie, 2011, 93 (4), pp.697-709. ⟨10.1016/j.biochi.2010.12.013⟩
ISSN: 0300-9084
DOI: 10.1016/j.biochi.2010.12.013⟩
Popis: International audience; Insulin increases muscle and fat cell glucose uptake by inducing the translocation of glucose transporter GLUT4 from intracellular compartments to the plasma membrane. Here, we have demonstrated that in 3T3-L1 adipocytes, DMSO at concentrations higher than 7.5% augmented cell surface GLUT4 levels in the absence and presence of insulin, but that at lower concentrations, DMSO only enhanced GLUT4 levels in insulin-stimulated cells. At a 5% concentration, DMSO also increased cell surface levels of the transferrin receptor and GLUT1. Glucose uptake experiments indicated that while DMSO enhanced cell surface glucose transporter levels, it also inhibited glucose transporter activity. Our studies further demonstrated that DMSO did not sensitize the adipocytes for insulin and that its effect on GLUT4 was readily reversible (t1/2∼12 min) and maintained in insulin-resistant adipocytes. An enhancement of insulin-induced GLUT4 translocation was not observed in 3T3-L1 preadipocytes and L6 myotubes, indicating cell specificity. DMSO did not enhance insulin signaling nor exocytosis of GLUT4 vesicles, but inhibited GLUT4 internalization. While other chemical chaperones (glycerol and 4-phenyl butyric acid) also acutely enhanced insulin-induced GLUT4 translocation, these effects were not mediated via changes in GLUT4 endocytosis. We conclude that DMSO is the first molecule to be described that instantaneously enhances insulin-induced increases in cell surface GLUT4 levels in adipocytes, at least in part through a reduction in GLUT4 endocytosis.
Databáze: OpenAIRE
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