Single-Particle Mobility Analysis Enables Ratiometric Detection of Cancer Markers under Darkfield Tracking Microscopy
| Autor: | Qian Yang, Jinhui Shang, Bin Xiong, Yueyue Tian, Decui Tang, Xiao-Bing Zhang, Yancao Chen |
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| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Microscopy Immunoconjugates medicine.diagnostic_test biology Biomolecule Metal Nanoparticles Tracking (particle physics) Antibodies Single Molecule Imaging Analytical Chemistry Carcinoembryonic Antigen Mobility analysis Carcinoembryonic antigen chemistry Colloidal gold Immunoassay medicine biology.protein Biomarkers Tumor Particle Humans Gold Biomedical engineering |
| Zdroj: | Analytical chemistry. 92(15) |
| ISSN: | 1520-6882 |
| Popis: | Here, we introduced a single-particle mobility analysis-based ratiometric strategy for quantitative detection of disease-related biomarkers using antibody-conjugated gold nanoparticles (AuNPs) as probes under darkfield tracking microscopy (DFTM). On the basis of the capability of discriminating nanoparticles with different hydrodynamic sizes and detecting the changes in hydrodynamic effect, single-particle mobility analysis enables us to determine the amount of aggregated and monodispersed nanoprobes for the sandwich-like immunoassay strategy, making it possible to quantify the biotargets by analyzing the relative changes in the aggregate-to-monomer ratio of nanoprobes. By using capture antibody and detection antibody conjugated AuNPs as nanoprobes, we demonstrated ratiometric detection of carcinoembryonic antigen (CEA) over a linear dynamic range from 50 to 750 pM, which is acceptable for clinical diagnostic analysis of CEA in tumor patients. This ratiometric detection technique exhibited excellent anti-interference ability in the presence of nonspecific proteins or complicated protein mixtures. It can be anticipated that this robust technique is promising for the accurate detection of disease biomarkers and other biomolecules for biochemical and diagnostic applications. |
| Databáze: | OpenAIRE |
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