Insights into the modes of action of tritium on the early-life stages of zebrafish, Danio rerio, using transcriptomic and proteomic analyses

Autor: Caroline Arcanjo, Sandrine Frelon, Olivier Armant, Luc Camoin, Stéphane Audebert, Virginie Camilleri, Isabelle Cavalié, Christelle Adam-Guillermin, Beatrice Gagnaire
Přispěvatelé: Laboratoire d'écotoxicologie des radionucléides (IRSN/PSE-ENV/SRTE/LECO), Service de recherche sur les transferts et les effets des radionucléides sur les écosystèmes (IRSN/PSE-ENV/SRTE), Institut de Radioprotection et de Sûreté Nucléaire (IRSN)-Institut de Radioprotection et de Sûreté Nucléaire (IRSN), Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de micro-irradiation, de métrologie et de dosimétrie neutrons (IRSN/PSE-SANTE/SDOS/LMDN), Service de dosimétrie (IRSN/PSE-SANTE/SDOS), This research was funded by IRSN and EDF S.A. (Electricit´e deFrance) through the GGP Environment program. The IBiSA MarseilleProteomic platform is funded by Institut Paoli-Calmettes, NationalInstitute of Cancer and Aix-Marseille University.
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Journal of Environmental Radioactivity
Journal of Environmental Radioactivity, 2023, 261, pp.107141. ⟨10.1016/j.jenvrad.2023.107141⟩
ISSN: 0265-931X
1879-1700
DOI: 10.1016/j.jenvrad.2023.107141⟩
Popis: International audience; In the environment, populations are exposed to different kinds of ionizing radiation. Little is known about their modes of action on non-human species, and whether or not they are similar for alpha, beta and gamma radiations, considered as the reference. In this context, tritium effects (beta emitter) under the form of tritiated water (HTO) were investigated in zebrafish, a common model in toxicology and ecotoxicology with a fully sequenced genome. Experiments were conducted on early life stages, considered to be highly sensitive to pollutants, byexposing eggs to 0.4 mGy/h of HTO until 10 days post fertilization. Tritium internalization was quantified, and effects were investigated using a combined approach of transcriptomic and proteomic analyses. Results highlighted similarities in the biological pathways affected by HTO by both techniques, such as defence response, muscle integrity and contraction, and potential visual alterations. These results correlated well with previous data obtained on earlier developmental stages (1 and 4 dpf). Interestingly, HTO effects were partly overlapping those obtained after gamma irradiation, underlying potential common modes of action. This study, therefore, brought a body of evidence on the effects of HTO observed at the molecular level on zebrafish larvae. Furtherstudies could investigate if the effects persist in adult organisms.
Databáze: OpenAIRE
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