Association of the angiotensinogen gene polymorphism with atherosclerosis and its risk traits in the Saudi population
Autor: | Nejat Mazhar, Maie Alshahid, Mohammed Al-Najai, Daisy Gueco, Samar Elhawari, Nada Al-Tassan, Editha Andres, Nduna Dzimiri, Asma I. Tahir, Paul Muiya |
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Rok vydání: | 2012 |
Předmět: |
Male
Genetic Linkage DNA Mutational Analysis Angiotensinogen Coronary Artery Disease Coronary Angiography Gastroenterology Gene Frequency Polymorphism (computer science) Risk Factors Odds Ratio Oligonucleotide Array Sequence Analysis education.field_of_study Middle Aged Arabs Primary hypertension Phenotype Gene-disease interactions Hypertension Female Cardiology and Cardiovascular Medicine Research Article medicine.medical_specialty Population Saudi Arabia Single-nucleotide polymorphism Real-Time Polymerase Chain Reaction Polymorphism Single Nucleotide Risk Assessment Internal medicine Angiotensinogen polymorphism Type 2 diabetes mellitus medicine Humans Genetic Predisposition to Disease Obesity education Genotyping Allele frequency Pleiotropy Chi-Square Distribution business.industry Haplotype Case-control study Odds ratio Endocrinology Logistic Models Diabetes Mellitus Type 2 Haplotypes Case-Control Studies business |
Zdroj: | BMC Cardiovascular Disorders |
ISSN: | 1471-2261 |
Popis: | Background Angiotensinogen (AGT) constitutes a central component of the renin-angiotensin system that controls the systemic blood pressure and several other cardiovascular functions and may play an important role in atherosclerosis pathways. In this study, we employed TaqMan genotyping assays to evaluate the role of 8 AGT variants in primary hypertension (HTN), type 2 diabetes mellitus (T2DM), and obesity as a possible trigger of coronary artery disease (CAD) in a population of 4615 angiographed native Saudi individuals. Methods Linkage analysis was done by using the Affymetrix Gene Chip array, sequencing by using the MegaBACE DNA analysis system and genotyping accomplished by TaqMan chemistry using the Applied Biosystem real-time Prism 7900HT Sequence Detection System. Results Six variants, rs2067853 GG [Odds ratio(95% Confidence Interval) = 1.44(1.17-1.78); p = 0.001], rs7079 [1.49(1.20-1.85); p 2 = 7.02; p = 0.0081) and another GGCGGAGT (χ2 = 5.10; p = 0.024), together with several of their derivatives were associated with HTN. T2DM was associated with two 8-mer haplotypes, GGTAGGAC (χ2 = 5.66; p = 0.017) and ATTGAGAC (χ2 = 5.93; p = 0.015), obesity with GGCGGAGT (χ2 = 9.49; p = 0.0021) and MI was linked to ATTGGGAC (χ2 = 6.68; p = 0.010) and GGTGGGAT (χ2 = 4.25; p = 0.039). Furthermore, several causative haplotypes were also shared among the risk traits as well as with CAD. Conclusion These results point to AGT as independently conferring risk for various cardiovascular traits, and possibly interacting with these traits in events leading to atherosclerosis. |
Databáze: | OpenAIRE |
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