Association of the angiotensinogen gene polymorphism with atherosclerosis and its risk traits in the Saudi population

Autor: Nejat Mazhar, Maie Alshahid, Mohammed Al-Najai, Daisy Gueco, Samar Elhawari, Nada Al-Tassan, Editha Andres, Nduna Dzimiri, Asma I. Tahir, Paul Muiya
Rok vydání: 2012
Předmět:
Male
Genetic Linkage
DNA Mutational Analysis
Angiotensinogen
Coronary Artery Disease
Coronary Angiography
Gastroenterology
Gene Frequency
Polymorphism (computer science)
Risk Factors
Odds Ratio
Oligonucleotide Array Sequence Analysis
education.field_of_study
Middle Aged
Arabs
Primary hypertension
Phenotype
Gene-disease interactions
Hypertension
Female
Cardiology and Cardiovascular Medicine
Research Article
medicine.medical_specialty
Population
Saudi Arabia
Single-nucleotide polymorphism
Real-Time Polymerase Chain Reaction
Polymorphism
Single Nucleotide

Risk Assessment
Internal medicine
Angiotensinogen polymorphism
Type 2 diabetes mellitus
medicine
Humans
Genetic Predisposition to Disease
Obesity
education
Genotyping
Allele frequency
Pleiotropy
Chi-Square Distribution
business.industry
Haplotype
Case-control study
Odds ratio
Endocrinology
Logistic Models
Diabetes Mellitus
Type 2

Haplotypes
Case-Control Studies
business
Zdroj: BMC Cardiovascular Disorders
ISSN: 1471-2261
Popis: Background Angiotensinogen (AGT) constitutes a central component of the renin-angiotensin system that controls the systemic blood pressure and several other cardiovascular functions and may play an important role in atherosclerosis pathways. In this study, we employed TaqMan genotyping assays to evaluate the role of 8 AGT variants in primary hypertension (HTN), type 2 diabetes mellitus (T2DM), and obesity as a possible trigger of coronary artery disease (CAD) in a population of 4615 angiographed native Saudi individuals. Methods Linkage analysis was done by using the Affymetrix Gene Chip array, sequencing by using the MegaBACE DNA analysis system and genotyping accomplished by TaqMan chemistry using the Applied Biosystem real-time Prism 7900HT Sequence Detection System. Results Six variants, rs2067853 GG [Odds ratio(95% Confidence Interval) = 1.44(1.17-1.78); p = 0.001], rs7079 [1.49(1.20-1.85); p 2 = 7.02; p = 0.0081) and another GGCGGAGT (χ2 = 5.10; p = 0.024), together with several of their derivatives were associated with HTN. T2DM was associated with two 8-mer haplotypes, GGTAGGAC (χ2 = 5.66; p = 0.017) and ATTGAGAC (χ2 = 5.93; p = 0.015), obesity with GGCGGAGT (χ2 = 9.49; p = 0.0021) and MI was linked to ATTGGGAC (χ2 = 6.68; p = 0.010) and GGTGGGAT (χ2 = 4.25; p = 0.039). Furthermore, several causative haplotypes were also shared among the risk traits as well as with CAD. Conclusion These results point to AGT as independently conferring risk for various cardiovascular traits, and possibly interacting with these traits in events leading to atherosclerosis.
Databáze: OpenAIRE