Limonene, a Chemical Compound Related to the Resistance of Eucalyptus Species to Austropuccinia psidii

Autor: José Eduardo Serrão, Marcus Alvarenga Soares, Edson Luiz Furtado, Roberto Antonio Rodella, André Luis Corrêa da Silva, José Cola Zanuncio, Renata Calciolari Rossi e Silva, Márcia O M Marques, Antônio José Vinha Zanuncio
Přispěvatelé: Universidade Estadual Paulista (Unesp), Univ Fed Rio Grande do Sul, Inst Agron Campinas, Universidade Federal de Viçosa (UFV)
Rok vydání: 2020
Předmět:
Zdroj: Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1943-7692
0191-2917
1861-0307
DOI: 10.1094/pdis-05-19-1002-re
Popis: Made available in DSpace on 2021-06-25T12:19:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-02-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) Instituto de Pesquisas e Estudos Florestais (IPEF) of Brazil Programa Cooperative sobre Protecao Florestal (PROTEF) The fungus Austropuccinia psidii is a major pathogen of Eucalyptus spp. that damages mainly early-stage leaves. Resistant clones are the most widely used control measure for the rust disease caused by A. psidii. Essential oils produced in the plant-host cells are associated with resistance. However, the chemical characteristics of Eucalyptus leaves at different stages of maturity, associated with resistance or susceptibility to A. psidii, need to be determined. The aims of this study were to characterize the chemical composition of essential oils in Eucalyptus leaves at three developmental stages of clones of three E. urophylla x E. grandis hybrids that exhibit different resistance levels to rust and to identify probable resistance-related compounds from them. The rust severity following inoculation and the quantity and quality of the essential oil extracted by hydrodistillation were determined at the first, third, and fifth leaf stages of the three clones. Identification of the compounds present in the essential oil was determined by gas chromatography coupled with mass spectrometry. No rust was observed on the three leaf stages of the resistant clone or on the fifth leaf stage of the susceptible clones. Limonene was found at high percentages in essential oils from the three leaf stages of the resistant clone and at low percentages in the susceptible clones. In vitro and in vivo tests indicated that low limonene percentages stimulated A. psidii urediniospore germination and were not fungitoxic, whereas high percentages caused complete inhibition of germination and degenerative changes in fungal reproductive structures. The limonene present in Eucalyptus leaves can be considered a chemical compound related to the rust resistance of Eucalyptus spp. Univ Estadual Paulista, Fac Ciencias Agron, Dept Prod Vegetal, BR-18610307 Botucatu, SP, Brazil Univ Fed Rio Grande do Sul, Dept Fitossanidade, BR-91540000 Porto Alegre, RS, Brazil Univ Estadual Paulista, Inst Biociencias Botucatu, Dept Bot, BR-18618000 Botucatu, SP, Brazil Inst Agron Campinas, Ctr Pesquisa Recursos Genet Vegetais, BR-13020902 Campinas, SP, Brazil Univ Fed Vicosa, Dept Engn Florestal, BR-36570900 Vicosa, MG, Brazil Univ Fed Vales Jequitinhonha & Mucuri, Programa Posgrad Prod Vegetal, BR-39100000 Diamantina, MG, Brazil Univ Fed Vicosa, Dept Biol Geral, BR-36570900 Vicosa, MG, Brazil Univ Fed Vicosa, Dept Entomol BIOAGRO, BR-36570900 Vicosa, MG, Brazil Univ Estadual Paulista, Fac Ciencias Agron, Dept Prod Vegetal, BR-18610307 Botucatu, SP, Brazil Univ Estadual Paulista, Inst Biociencias Botucatu, Dept Bot, BR-18618000 Botucatu, SP, Brazil CAPES: 001
Databáze: OpenAIRE
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