Change in the Clonal Structure of Carbapenem not Susceptible Acinetobacter baumannii Isolated from the Blood Culture of Patients with Hematological Malignancies
| Autor: | I.N. Frolova, A.G. Korobova, G.A. Klyasova, S. A. Khrulnova, A. V. Fedorova |
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| Rok vydání: | 2020 |
| Předmět: |
clone (Java method)
Imipenem Carbapenem Biology Microbiology Meropenem 03 medical and health sciences Virology Genetics medicine Blood culture Allele Molecular Biology Gene 030304 developmental biology 0303 health sciences medicine.diagnostic_test 030306 microbiology General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Acinetobacter baumannii Infectious Diseases medicine.drug |
| Zdroj: | Molecular Genetics, Microbiology and Virology. 35:145-151 |
| ISSN: | 1934-841X 0891-4168 |
| DOI: | 10.3103/s0891416820030039 |
| Popis: | The emergence and dissemination of successful clones of A. baumannii is a serious problem for the medical community. To investigate the clonal diversity of A. baumannii isolated from blood culture of patients with hematological malignancies. The study included A. baumannii isolated from blood culture of patients in seven Russian hospitals (2003–2017). Two multiplex PCRs were performed to selectively amplify group 1 or group 2 alleles of the ompA, csuE, and blaOXA-51-like genes. Isolates were assigned to one of the sequence groups (G1–G14) according to allelic profiles. A total of 96 A. baumannii were examined, including 77 (80.2%) not susceptible to meropenem and/or imipenem. The genes of acquired carbapenemases were detected in 79.2% (61/77) carbapenem not susceptible isolates. The genes encoding metallo-β-lactamases (blaNDM, blaVIM, and blaIMP) were not revealed. The prevalent carbapenemase genes were blaOXA-24/40-like (45.9%) and blaOXA-23-like (45.9%), followed by blaOXA-58-like (6.6%), and coharboring blaOXA-24/40-like and blaOXA-23-like in one isolate (1.6%). During the study period, the rate of blaOXA-23-like-carrying A. baumannii increased from 30 (2003–2010) to 53.7% (2011–2017), whereas A. baumannii harboring blaOXA-24/40-like decreased from 50% (2003–2010) to 43.9% (2011–2017). The majority of A. baumannii carrying acquired carbapenemase genes (62.3%) belonged to the G1 group (international clone II, IC II), which is widespread in the world. G1 (IC II) isolates harboring blaOXA-23-like were not detected during 2003—2010, whereas in 2011–2017 G1 (IC II) A. baumannii carrying blaOXA-23-like accounted for 86.4%. During the study period, there was a slight decrease in the rate of blaOXA-24/40-like-carrying A. baumannii from 70 (2003–2010) to 53.7% (2011–2017). An increase of G1 (ICII) A. baumannii harboring blaOXA-23-like was observed during recent years (2011–2017). |
| Databáze: | OpenAIRE |
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