A genetic effect of altered gravity: mutations induced by simulated hypogravity and hypergravity in microsatellite sequences of human tumor cells
| Autor: | Kanji Ishizaki, Zhen Bo Han, Mituo Ikenaga, Kimiko Nishizawa, Tomohisa Kato, Takeshi Todo |
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| Rok vydání: | 1999 |
| Předmět: |
Hypergravity
Mutation Gravity Altered Health Toxicology and Mutagenesis X-Rays Mutant Biology medicine.disease_cause Molecular biology Polymerase Chain Reaction Gene expression Genetics medicine Tumor Cells Cultured Hypogravity Microsatellite Humans Tetradecanoylphorbol Acetate Allele Molecular Biology Clinostat DNA Primers Microsatellite Repeats |
| Zdroj: | Mutation research. 426(1) |
| ISSN: | 0027-5107 |
| Popis: | To determine the possible genetic effects of gravity alterations, we analyzed mutation induction in microsatellite sequences of human tumor cells treated with simulated hypogravity provided by a clinostat or hypergravity by a centrifuge. Microsatellite mutations were detected as changes in the size of polymerase chain reaction (PCR)-amplified allelic markers. The frequencies of mutant clones in cultures treated with simulated hypogravity for 24 or 48 h were almost the same as those of controls, but after 72 h of treatment, the mutant frequencies had increased significantly in all three microsatellite loci examined. Significantly higher mutant frequencies were similarly detected in cultures treated for 72 h with a hypergravity condition as low as 18× g , but not detected in 24 or 48 h treated cultures. These findings clearly show that gravity alterations that last for 3 days can induce microsatellite mutations in human cells. A genetic effect of gravity change, therefore, is established for the first time. Moreover, high frequencies of microsatellite mutations were induced by 12- O -tetradecanoylphorbol-13-acetate (TPA) which activates protein kinase C-mediated signal transduction pathways and causes genetic instability. These findings suggest that gravity change induces microsatellite mutations by modulating the pattern of gene expression involved in signal transduction pathways. |
| Databáze: | OpenAIRE |
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