RENAL LOCALIZATION OF TRITIATED STREPTOCOCCAL POLYPEPTIDE

Autor:	S. C. Elliott, C. K. Williamson
Rok vydání:	1966
Předmět:	Electrophoresis Male Aspartic Acid Nephritis Histology Proline Chromatography Paper Streptococcus pyogenes Chemistry Glycine Tritium medicine.disease_cause Mice Paper chromatography Glutamates Biochemistry medicine Animals Autoradiography Amino Acids Anatomy Peptides Ultracentrifugation
Zdroj:	Journal of Histochemistry & Cytochemistry. 14:491-494
ISSN:	1551-5044 0022-1554
DOI:	10.1177/14.6.491
Popis:	Certain strains of Streptococcus pyogenes, Type 12 elaborate a polypeptide which is nephritogenic in experimental animals. It appeared to be a pure, nonmigrating component by paper chromatography, to be electrophoretically pure when analyzed by the Spinco electrophoretic system, and to be homogeneous by ultracentrifugal analysis. Quantitative amino acid analyses of acid-hydrolyzed samples revealed 19 amino acids and trace amounts of five unidentified components. Glycine, proline, alanine, glutamic acid and aspartic acid accounted for 70% of the amino acids found. Wilzbach tritiated samples were injected intraperitoneally into 20-g C57B1 male mice. Radioautographic evidence revealed that a high percentage of the injected material concentrated in renal tissue.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb57ed7ef878c0c8356a5e377362d1b9 https://doi.org/10.1177/14.6.491 Zobrazit plný text záznamu