Monocyte chemo attractant protein-1 and interleukin-8 levels in urine and serum of patients with hemolytic uremic syndrome
| Autor: | Thea J A M van der Velden, Frank Preyers, H.B.P.M. Dijkman, Lambertus P. van den Heuvel, Victor W.M. van Hinsbergh, Leo A. H. Monnens, Petra A Van Setten, Karel J.M. Assmann |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Male
medicine.medical_specialty Chemokine Immunologische ontstekingsprocessen in de nier Renal glomerulus Neutrophils Urinary system Biopsy urologic and male genital diseases Kidney Monocytes Reference Values Internal medicine Inflammatory reactions in the kidneys medicine Humans Interleukin 8 Child Chemokine CCL2 biology Monocyte Interleukin-8 Infant Glomerulonephritis Epidemiologie en pathogenese van het hemolytisch uremisch syndroom medicine.disease Epidemiology and pathogenesis of the hemolytic uremic syndrome Blood Cell Count Endocrinology medicine.anatomical_structure Child Preschool Pediatrics Perinatology and Child Health Immunology Hemolytic-Uremic Syndrome biology.protein Anuria Female medicine.symptom Tumorimmunology Biomarkers |
| Zdroj: | Pediatric Research, 43, 759-767 Pediatric Research, 43, 6, pp. 759-767 |
| ISSN: | 0031-3998 |
| Popis: | The epidemic form of the hemolytic uremic syndrome (HUS) in children is hallmarked by endothelial cell damage, most predominantly displayed by the glomerular capillaries. The influx of mononuclear (MO) and polymorphonuclear cells (PMNs) into the glomeruli may be an important event in the initiation, prolongation, and progression of glomerular endothelial cell damage in HUS patients. The molecular mechanisms for the recruitment of these leukocytes into the kidney are unclear, but monocyte chemoattractant protein-1 (MCP-1) and IL-8 are suggested to be prime candidates. In this study, we analyzed the presence of both chemokines in 24-h urinary (n = 15) and serum (n = 14) samples of HUS children by specific ELISAs. Furthermore, kidney biopsies of three different HUS children were examined for MO and PMN cell infiltration by histochemical techniques and electron microscopy. Whereas the chemokines MCP-1 and IL-8 were present in only very limited amounts in urine of 17 normal control subjects, serial samples of HUS patients demonstrated significantly elevated levels of both chemokines. HUS children with anuria showed higher initial and maximum chemokine levels than their counterparts without anuria. A strong positive correlation was observed between urinary MCP-1 and IL-8 levels. Whereas initial serum IL-8 levels were significantly increased in HUS children, serum MCP-1 levels were only slightly elevated compared with serum MCP-1 in control children. No correlation was found between urinary and serum chemokine concentrations. Histologic and EM studies of HUS biopsy specimens clearly showed the presence of MOs and to a lesser extent of PMNs in the glomeruli. The present data suggest an important local role for MOs and PMNs in the process of glomerular endothelial-cell damage. The chemokines MCP-1 and IL-8 may possibly be implicated in the pathogenesis of HUS through the recruitment and activation of MOs and PMNs, respectively. |
| Databáze: | OpenAIRE |
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