The evaluation of oxidative DNA damage in children with brain damage using 8-hydroxydeoxyguanosine levels
| Autor: | Yusaku Miyamoto, Yasushi Koitabashi, Hiroshi Yamauchi, Miho Fukuda, Hiroshi Murakami, Hitoshi Yamamoto, Masahito Aminaka, Noriko Kamiyama |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Pathology medicine.medical_specialty Adolescent DNA damage Urinary system Encephalopathy Central nervous system Physiology Status epilepticus Brain damage medicine.disease_cause Statistics Nonparametric Cerebrospinal fluid Developmental Neuroscience medicine Humans Longitudinal Studies Child Chromatography High Pressure Liquid Brain Diseases business.industry Infant Newborn Deoxyguanosine Infant General Medicine medicine.disease Oxidative Stress medicine.anatomical_structure 8-Hydroxy-2'-Deoxyguanosine Case-Control Studies Child Preschool Pediatrics Perinatology and Child Health Female Neurology (clinical) medicine.symptom business Oxidative stress DNA Damage |
| Zdroj: | Brain and Development. 30:131-136 |
| ISSN: | 0387-7604 |
| DOI: | 10.1016/j.braindev.2007.07.005 |
| Popis: | Urinary and cerebrospinal fluid (CSF) levels of 8-hydroxydeoxyguanosine (8-OHdG) were examined to estimate the relevance of oxidative stress in children with brain damage. Urinary 8-OHdG levels were measured in 51 children with various forms of central nervous system (CNS) disorders (status epilepticus [SE], hypoxic-ischemic encephalopathy [HIE], CNS infections and chronic epilepsy) and these levels were compared with those in 51 healthy children. CSF 8-OHdG levels were measured in 25 children with brain damage and in 19 control subjects. In addition, urinary and CSF levels of 8-OHdG were compared between the children with brain damage and healthy children. Finally, the relationship between urinary and CSF levels of 8-OHdG was determined in 12 children that provided both urinary and CSF samples. Our results showed that urinary 8-OHdG levels in children with HIE and CNS infections were higher than those of controls (Steel test; p0.05 and p0.05, respectively) and that CSF 8-OHdG levels were higher in children with SE, HIE, and CNS infections than in control subjects (Steel test; p0.01, 0.05 and 0.05, respectively). In addition, a positive correlation between the levels of urinary and CSF 8-OHdG was noted in the 12 children that provided both CSF and urinary samples (Spearman's rank correlation; rho = 0.82, p0.01). Further, we observed changes in the urinary 8-OHdG in a patient with HHV-6 encephalopathy, and found that the changes correlated well with the patient's clinical condition. These results suggest that oxidative stress is strongly related to acute brain damage in children, and that 8-OHdG is a useful marker of brain damage. Therefore, repeated measurements of urinary 8-OHdG may be helpful in estimating the extent of brain damage. |
| Databáze: | OpenAIRE |
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