Su(var)2-10 and the SUMO Pathway Link piRNA-Guided Target Recognition to Chromatin Silencing
| Autor: | Katalin Fejes Tóth, Maria Ninova, Baira K Godneeva, Yenyu Chen, Alexei A. Aravin, Alicia K. Rogers, Yicheng Luo |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Small RNA
endocrine system Transcription Genetic Chromatin silencing Piwi-interacting RNA Biology Article 03 medical and health sciences 0302 clinical medicine Transcriptional regulation Gene silencing Animals Drosophila Proteins Gene Silencing RNA Small Interfering Molecular Biology Transcription factor 030304 developmental biology Cell Nucleus 0303 health sciences urogenital system Cell Biology Protein Inhibitors of Activated STAT Chromatin Cell biology Drosophila melanogaster Gene Expression Regulation Histone methyltransferase Argonaute Proteins DNA Transposable Elements Small Ubiquitin-Related Modifier Proteins 030217 neurology & neurosurgery Protein Binding |
| Zdroj: | Mol Cell |
| ISSN: | 1097-4164 |
| Popis: | Regulation of transcription is the main mechanism responsible for precise control of gene expression. Whereas the majority of transcriptional regulation is mediated by DNA-binding transcription factors that bind to regulatory gene regions, an elegant alternative strategy employs small RNA guides, Piwi-interacting RNAs (piRNAs) to identify targets of transcriptional repression. Here, we show that in Drosophila the small ubiquitin-like protein SUMO and the SUMO E3 ligase Su(var)2-10 are required for piRNA-guided deposition of repressive chromatin marks and transcriptional silencing of piRNA targets. Su(var)2-10 links the piRNA-guided target recognition complex to the silencing effector by binding the piRNA/Piwi complex and inducing SUMO-dependent recruitment of the SetDB1/Wde histone methyltransferase effector. We propose that in Drosophila, the nuclear piRNA pathway has co-opted a conserved mechanism of SUMO-dependent recruitment of the SetDB1/Wde chromatin modifier to confer repression of genomic parasites. |
| Databáze: | OpenAIRE |
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