Antagonist Xist and Tsix co-transcription during mouse oogenesis and maternal Xist expression during pre-implantation development calls into question the nature of the maternal imprint on the X chromosome
| Autor: | Nathalie Beaujean, Philip Avner, Céline Morey, Jane Lynda Deuve, Amélie Bonnet-Garnier |
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| Přispěvatelé: | Génétique Moléculaire Murine, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), This work was supported by a Marie Curie ERG grant to CM (#224945), by recurrent funding from the Institut Pasteur and the CNRS (Center National pour la Recherche Scientifique), and by grants from the ANR (Agence Nationale pour la Recherche), the Epigenome Network of Excellence, the REVIVE Labex and from the Fondation Louis D. Institut de France to PA. JLD was supported by a postdoctoral fellowship from the ARC (Association pour la Recherche contre le Cancer)., We thank Angélique Vincent for her help in collecting Pwk/129Sv pre-implantation embryos. We thank Frank Feuerbach for advices on polyA+RNA pull-down, Khalil Mazouni for Taf11 primers and Franck Coumailleau for the gift of Drosophila larvae. We thank Michel Cohen-Tannoudji and Sandrine Vandormael-Pournin for their insights on gene expression during oogenesis, for critical reading of the manuscript and for the gift of ovary sections. We thank Claire Rougeulle for critical reading of the manuscript. We thank Martine Chebrout for her help in collecting SN and NSN oocytes. We thank the Center d'Immunologie Humaine (CIH-Human Immunology Center) for allowing and facilitating the use of the Biomark thermocycler. We thank Ann Holmberg from the QLUCORE company for her help with the analysis of single-cell RT-qPCR results. We thank Katja Wassmann, Damien Cladière and Soraya Sin for their help in finalising the last experiments., Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Cancer Research
mouse oogenesis MESH: Oocytes/metabolism [SDV]Life Sciences [q-bio] souris Mice long non-coding RNAs Oogenesis X Chromosome Inactivation Transcription (biology) MESH: Gene Expression Regulation Developmental Biologie de la reproduction chromosome x single-cell analysis MESH: Oocytes/growth & development MESH: Oogenesis/genetics MESH: Animals Imprinting (psychology) [SDV.BDD]Life Sciences [q-bio]/Development Biology X chromosome Genetics Reproductive Biology Biologie du développement mouse pre-implantation development Gene Expression Regulation Developmental Embryo Development Biology MESH: Embryonic Development/genetics MESH: RNA Long Noncoding/biosynthesis Female RNA Long Noncoding MESH: RNA Long Noncoding/genetics MESH: X Chromosome Inactivation/genetics imprinting transcription MESH: Genomic Imprinting/genetics Research Paper X Chromosome Embryonic Development [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology X-inactivation Genomic Imprinting MESH: X Chromosome/genetics Animals Molecular Biology MESH: Mice ovogénèse MESH: Embryo Mammalian Embryo Mammalian Oocytes XIST empreinte génétique Tsix x-inactivation Genomic imprinting MESH: Female |
| Zdroj: | Epigenetics Epigenetics, Taylor & Francis, 2015, 10 (10), pp.931-942. ⟨10.1080/15592294.2015.1081327⟩ Epigenetics, 2015, 10 (10), pp.931-942. ⟨10.1080/15592294.2015.1081327⟩ Epigenetics 10 (10), 931-942. (2015) |
| ISSN: | 1559-2294 1559-2308 |
| Popis: | International audience; During the first divisions of the female mouse embryo, the paternal X-chromosome is coated by Xist non-coding RNA and gradually silenced. This imprinted X-inactivation principally results from the apposition, during oocyte growth, of an imprint on the X-inactivation master control region: the X-inactivation center (Xic). This maternal imprint of yet unknown nature is thought to prevent Xist upregulation from the maternal X (XM) during early female development. In order to provide further insight into the XM imprinting mechanism, we applied single-cell approaches to oocytes and pre-implantation embryos at different stages of development to analyze the expression of candidate genes within the Xic. We show that, unlike the situation pertaining in most other cellular contexts, in early-growing oocytes, Xist and Tsix sense and antisense transcription occur simultaneously from the same chromosome. Additionally, during early development, Xist appears to be transiently transcribed from the XM in some blastomeres of late 2-cell embryos concomitant with the general activation of the genome indicating that XM imprinting does not completely suppress maternal Xist transcription during embryo cleavage stages. These unexpected transcriptional regulations of the Xist locus call for a re-evaluation of the early functioning of the maternal imprint on the X-chromosome and suggest that Xist/Tsix antagonist transcriptional activities may participate in imprinting the maternal locus as described at other loci subject to parental imprinting. |
| Databáze: | OpenAIRE |
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