Production and properties of a factor X–cellulose-binding domain fusion protein
| Autor: | Z. Assouline, H. Shen, Douglas G. Kilburn, R. A. J. Warren |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Immobilized enzyme
Recombinant Fusion Proteins Blotting Western Molecular Sequence Data Bioengineering Kidney Transfection digestive system Biochemistry Chromatography Affinity Cell Line chemistry.chemical_compound Affinity chromatography Cricetinae Animals Amino Acid Sequence Cloning Molecular Cellulose Molecular Biology Binding Sites Chromatography Base Sequence Binding protein Factor X Substrate (chemistry) Cellulose binding Fusion protein digestive system diseases surgical procedures operative Chromogenic Compounds chemistry Biotechnology |
| Zdroj: | "Protein Engineering, Design and Selection". 6:787-792 |
| ISSN: | 1741-0134 1741-0126 |
| DOI: | 10.1093/protein/6.7.787 |
| Popis: | A fusion protein, FX-CBD Cex , which comprises factor X with a cellulose-binding domain (CBD Cex ) fused to its C-terminus, was produced in BHK cells. It was purified from the culture medium by affinity chromatography on cellulose. FX-CBD Cex could be activated to FXa-CBD Cex with Russell viper venom. FXa-CBD Cex was as active as FXa against a chromogenic substrate and against proteins confining the Ile-Glu-Gly-Arg sequence hydrolysed by FXa. FXa-CBD Cex retained its activity when adsorbed to cellulose |
| Databáze: | OpenAIRE |
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